BMRB Entry 10231
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10231
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Title: Solution structure of the C2H2 type zinc finger (region 598-626) of human B-cell lymphoma 6 protein
Deposition date: 2008-08-19 Original release date: 2009-08-19
Authors: Tochio, N.; Tomizawa, T.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Tomizawa, T.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc finger (region 598-626) of human B-cell lymphoma 6 protein" . ., .-..
Assembly members:
zf-C2H2, UNP residues 598-626, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
zf-C2H2, UNP residues 598-626: GSSGSSGGEKPYKCETCGAR
FVQVAHLRAHVLIHTGSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 146 |
| 15N chemical shifts | 32 |
| 1H chemical shifts | 219 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-C2H2, UNP residues 598-626 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, zf-C2H2, UNP residues 598-626 42 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | GLU | LYS | ||||
| 2 | PRO | TYR | LYS | CYS | GLU | THR | CYS | GLY | ALA | ARG | ||||
| 3 | PHE | VAL | GLN | VAL | ALA | HIS | LEU | ARG | ALA | HIS | ||||
| 4 | VAL | LEU | ILE | HIS | THR | GLY | SER | GLY | PRO | SER | ||||
| 5 | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-C2H2, UNP residues 598-626, [U-13C; U-15N], 1.0 ± 0.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 0.05 mM; IDA 1 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
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