BMRB Entry 15522
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15522
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Title: Solution structure of pUL89(580-600) from Human Cytomegalovirus at pH 6.8.
Deposition date: 2007-10-12 Original release date: 2007-10-17
Authors: Couvreux, Anthony; Champier, Gael; Marquant, Rodrigue; Hantz, Sebastien; Alain, Sophie; Morellet, Nelly; Bouaziz, Serge
Citation: Couvreux, Anthony; Champier, Gael; Marquant, Rodrigue; Hantz, Sebastien; Alain, Sophie; Morellet, Nelly; Bouaziz, Serge. "Solution structure of the small terminase subunit interaction domain of pUL89 within the Human Cytomegalovirus terminase complex." Biochem. J. ., .-..
Assembly members:
pUL89(580-600), polymer, 21 residues, 2403.760 Da.
Natural source: Common Name: Human herpesvirus 5 Taxonomy ID: 10359 Superkingdom: Viruses Kingdom: not available Genus/species: Cytomegalovirus Human herpesvirus 5
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
pUL89(580-600): GRDKALAVEQFISRFNSGYI
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 300 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 21 residues - 2403.760 Da.
| 1 | GLY | ARG | ASP | LYS | ALA | LEU | ALA | VAL | GLU | GLN | ||||
| 2 | PHE | ILE | SER | ARG | PHE | ASN | SER | GLY | TYR | ILE | ||||
| 3 | LYS |
Samples:
sample: pUL89(580-600) 1.4 mM; TFE, [U-99% 2H], 30%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 280 K
sample_conditions_2: pH: 2.8; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample | isotropic | sample_conditions_1 |
Software:
FELIX v98.0, Accelrys Software Inc. - chemical shift assignment, peak picking, processing
NMR spectrometers:
- Bruker DRX 600 MHz