BMRB Entry 15587
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15587
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Title: 1H, 13C and 15N resonance assignment of Urm1 from Trypanosoma.brucei PubMed: 19636925
Deposition date: 2007-12-11 Original release date: 2008-03-31
Authors: Zhang, Wen; Ding, Husheng; Zhang, Jiahai; Tu, xiaoming
Citation: Zhang, Wen; Ding, Husheng; Zhang, Jiahai; Tu, Xiaoming. "1H, 13C and 15N resonance assignment of Urm1 from Trypanosoma brucei" Biomol. NMR Assignments 2, 59-60 (2008).
Assembly members:
ubiquitin-related_modifier, polymer, 110 residues, Formula weight is not available
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ubiquitin-related_modifier: MSNHNHITVQFAGGCELLFA
KQTSLQLDGVVPTGTNLNGL
VQLLKTNYVKERPDLLVDQT
GQTLRPGILVLVNSCDAEVV
GGMDYVLNDGDTVEFISTLH
GGLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 397 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 617 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 110 residues - Formula weight is not available
| 1 | MET | SER | ASN | HIS | ASN | HIS | ILE | THR | VAL | GLN | |
| 2 | PHE | ALA | GLY | GLY | CYS | GLU | LEU | LEU | PHE | ALA | |
| 3 | LYS | GLN | THR | SER | LEU | GLN | LEU | ASP | GLY | VAL | |
| 4 | VAL | PRO | THR | GLY | THR | ASN | LEU | ASN | GLY | LEU | |
| 5 | VAL | GLN | LEU | LEU | LYS | THR | ASN | TYR | VAL | LYS | |
| 6 | GLU | ARG | PRO | ASP | LEU | LEU | VAL | ASP | GLN | THR | |
| 7 | GLY | GLN | THR | LEU | ARG | PRO | GLY | ILE | LEU | VAL | |
| 8 | LEU | VAL | ASN | SER | CYS | ASP | ALA | GLU | VAL | VAL | |
| 9 | GLY | GLY | MET | ASP | TYR | VAL | LEU | ASN | ASP | GLY | |
| 10 | ASP | THR | VAL | GLU | PHE | ILE | SER | THR | LEU | HIS | |
| 11 | GLY | GLY | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: ubiquitin-related modifier 0.8-1 mM; NaH2PO4 50 mM; NaCL 100 mM; EDTA 1.5 mM; D2O, [U-100% 2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts