BMRB Entry 16699
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16699
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Title: 1H Chemical Shift Assignments for gH617-644 fusion peptide from HSV-1 gH protein PubMed: 20348105
Deposition date: 2010-02-01 Original release date: 2010-05-05
Authors: Russo, Luigi; Isernia, Carla; Galdiero, Stefania; Falanga, Annarita; Vitiello, Mariateresa; Raiola, Luca; Pedone, Carlo; Galdiero, Massimiliano
Citation: Galdiero, Stefania; Falanga, Annarita; Vitiello, Mariateresa; Raiola, Luca; Russo, Luigi; Pedone, Carlo; Isernia, Carla; Galdiero, Massimiliano. "The Presence of a Single N-terminal Histidine Residue Enhances the Fusogenic Properties of a Membranotropic Peptide Derived from Herpes Simplex Virus Type 1 Glycoprotein H." J. Biol. Chem. 285, 17123-17136 (2010).
Assembly members:
HSV-1_gH_protein,_gH617-644_fusion_peptide, polymer, 28 residues, 3167.6 Da.
Natural source: Common Name: Human herpesvirus 1 Taxonomy ID: 10298 Superkingdom: Viruses Kingdom: not available Genus/species: Simplexvirus Human herpesvirus 1
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HSV-1_gH_protein,_gH617-644_fusion_peptide: VEVLAQQTHGLASTLTRWAH
YNALIRAF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 207 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HSV-1 gH protein, gH617-644 fusion peptide | 1 |
Entities:
Entity 1, HSV-1 gH protein, gH617-644 fusion peptide 28 residues - 3167.6 Da.
| 1 | VAL | GLU | VAL | LEU | ALA | GLN | GLN | THR | HIS | GLY | ||||
| 2 | LEU | ALA | SER | THR | LEU | THR | ARG | TRP | ALA | HIS | ||||
| 3 | TYR | ASN | ALA | LEU | ILE | ARG | ALA | PHE |
Samples:
sample_gH617-644: HSV-1 gH protein, gH617-644 fusion peptide 1 mM; TFE, [U-99% 2H], 80 % v/v; H2O 20 % v/v
sample_condition_gH617-644: pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_gH617-644 | isotropic | sample_condition_gH617-644 |
| 2D 1H-1H NOESY | sample_gH617-644 | isotropic | sample_condition_gH617-644 |
| 2D DQF-COSY | sample_gH617-644 | isotropic | sample_condition_gH617-644 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
Molmol, Koradi, Billeter and Wuthrich - data analysis
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Varian Unity 500 MHz