BMRB Entry 17985
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17985
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Title: Structure of BST-2/Tetherin Transmembrane Domain PubMed: 22072710
Deposition date: 2011-10-07 Original release date: 2011-12-12
Authors: Skasko, Mark; Wang, Yan; Tian, Ye; Tokarev, Andrey; Munguia, Jason; Ruiz, Autumn; Stephens, Edward; Opella, Stanley; Guatelli, John
Citation: Skasko, Mark; Wang, Yan; Tian, Ye; Tokarev, Andrey; Munguia, Jason; Ruiz, Autumn; Stephens, Edward; Opella, Stanley; Guatelli, John. "HIV-1 Vpu protein antagonizes innate restriction factor BST-2 via lipid-embedded helix-helix interactions." J. Biol. Chem. 287, 58-67 (2012).
Assembly members:
BST-2, polymer, 24 residues, 2529.350 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
BST-2: LLLGIGILVLLIIVILGVPL
IIFT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 24 |
| 15N chemical shifts | 23 |
| 1H chemical shifts | 23 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BST-2 | 1 |
Entities:
Entity 1, BST-2 24 residues - 2529.350 Da.
| 1 | LEU | LEU | LEU | GLY | ILE | GLY | ILE | LEU | VAL | LEU | ||||
| 2 | LEU | ILE | ILE | VAL | ILE | LEU | GLY | VAL | PRO | LEU | ||||
| 3 | ILE | ILE | PHE | THR |
Samples:
sample_1: BST-2, [U-100% 15N], 0.5 mM; H2O, [U-100% 15N], 90%; D2O, [U-100% 15N], 10%
sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA05679 BAD96844 BAF83788 BAG64608 |
| GB | AAH33873 ACX46128 ADI58594 ADI58595 EAW84602 |
| REF | NP_001266147 NP_001266560 NP_004326 |
| SP | Q10589 |
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