BMRB Entry 18376
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18376
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Title: Free MTIP(61-204) from Plasmodium falciparum PubMed: 22932904
Deposition date: 2012-04-03 Original release date: 2012-04-16
Authors: Douse, Christopher
Citation: Douse, Christopher; Green, Judith; Salgado, Paula; Simpson, Peter; Thomas, Jemima; Langsley, Gordon; Holder, Anthony; Tate, Edward; Cota, Ernesto. "Regulation of the Plasmodium motor complex: phosphorylation of myosin A tail-interacting protein (MTIP) loosens its grip on MyoA." J. Biol. Chem. 287, 36968-36977 (2012).
Assembly members:
MTIP(61-204), polymer, 154 residues, 17605 Da.
Natural source: Common Name: malaria parasite P. falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MTIP(61-204): RGSHHHHHHGSVADIQQLEE
KVDESDVRIYFNEKSSGGKI
SIDNASYNARKLGLAPSSID
EKKIKELYGDNLTYEQYLEY
LSICVHDKDNVEELIKMFAH
FDNNCTGYLTKSQMKNILTT
WGDALTDQEAIDALNAFSSE
DNIDYKLFCEDILQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 137 |
| 1H chemical shifts | 137 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MTIP(61-204) | 1 |
Entities:
Entity 1, MTIP(61-204) 154 residues - 17605 Da.
| 1 | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | GLY | ||||
| 2 | SER | VAL | ALA | ASP | ILE | GLN | GLN | LEU | GLU | GLU | ||||
| 3 | LYS | VAL | ASP | GLU | SER | ASP | VAL | ARG | ILE | TYR | ||||
| 4 | PHE | ASN | GLU | LYS | SER | SER | GLY | GLY | LYS | ILE | ||||
| 5 | SER | ILE | ASP | ASN | ALA | SER | TYR | ASN | ALA | ARG | ||||
| 6 | LYS | LEU | GLY | LEU | ALA | PRO | SER | SER | ILE | ASP | ||||
| 7 | GLU | LYS | LYS | ILE | LYS | GLU | LEU | TYR | GLY | ASP | ||||
| 8 | ASN | LEU | THR | TYR | GLU | GLN | TYR | LEU | GLU | TYR | ||||
| 9 | LEU | SER | ILE | CYS | VAL | HIS | ASP | LYS | ASP | ASN | ||||
| 10 | VAL | GLU | GLU | LEU | ILE | LYS | MET | PHE | ALA | HIS | ||||
| 11 | PHE | ASP | ASN | ASN | CYS | THR | GLY | TYR | LEU | THR | ||||
| 12 | LYS | SER | GLN | MET | LYS | ASN | ILE | LEU | THR | THR | ||||
| 13 | TRP | GLY | ASP | ALA | LEU | THR | ASP | GLN | GLU | ALA | ||||
| 14 | ILE | ASP | ALA | LEU | ASN | ALA | PHE | SER | SER | GLU | ||||
| 15 | ASP | ASN | ILE | ASP | TYR | LYS | LEU | PHE | CYS | GLU | ||||
| 16 | ASP | ILE | LEU | GLN |
Samples:
sample_1: MTIP(61-204), [U-98% 13C; U-98% 15N], 0.5 mM; MOPS buffer 20 mM; sodium chloride 50 mM; TCEP 1 mM; sodium azide 0.005%; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 18377 |
| PDB | |
| EMBL | CDO65736 |
| GB | AAN36529 ETW17315 ETW29792 ETW35145 ETW41309 |
| REF | XP_001350849 XP_012764323 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts