BMRB Entry 18627
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18627
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Calcium free, Myristoylated Visinin-like protein 3 PubMed: 23250791
Deposition date: 2012-07-31 Original release date: 2013-02-14
Authors: Li, Congmin; Ames, James
Citation: Li, Congmin; Ames, James. "H, 13C, and 15N chemical shift assignments of neuronal calcium sensor protein, hippocalcin." Biomol. NMR Assignments 8, 63-66 (2014).
Assembly members:
VILIP-3 monomer, polymer, 193 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
VILIP-3 monomer: MGKQNSKLRPEVLQDLRENT
EFTDHELQEWYKGFLKDCPT
GHLTVDEFKKIYANFFPYGD
ASKFAEHVFRTFDTNGDGTI
DFREFIIALSVTSRGKLEQK
LKWAFSMYDLDGNGYISRSE
MLEIVQAIYKMVSSVMKMPE
DESTPEKRTDKIFRQMDTNN
DGKLSLEEFIRGAKSDPSIV
RLLQCDPSSASQF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 599 |
| 15N chemical shifts | 174 |
| 1H chemical shifts | 1103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VILIP-3 monomer | 1 |
Entities:
Entity 1, VILIP-3 monomer 193 residues - Formula weight is not available
| 1 | MET | GLY | LYS | GLN | ASN | SER | LYS | LEU | ARG | PRO | ||||
| 2 | GLU | VAL | LEU | GLN | ASP | LEU | ARG | GLU | ASN | THR | ||||
| 3 | GLU | PHE | THR | ASP | HIS | GLU | LEU | GLN | GLU | TRP | ||||
| 4 | TYR | LYS | GLY | PHE | LEU | LYS | ASP | CYS | PRO | THR | ||||
| 5 | GLY | HIS | LEU | THR | VAL | ASP | GLU | PHE | LYS | LYS | ||||
| 6 | ILE | TYR | ALA | ASN | PHE | PHE | PRO | TYR | GLY | ASP | ||||
| 7 | ALA | SER | LYS | PHE | ALA | GLU | HIS | VAL | PHE | ARG | ||||
| 8 | THR | PHE | ASP | THR | ASN | GLY | ASP | GLY | THR | ILE | ||||
| 9 | ASP | PHE | ARG | GLU | PHE | ILE | ILE | ALA | LEU | SER | ||||
| 10 | VAL | THR | SER | ARG | GLY | LYS | LEU | GLU | GLN | LYS | ||||
| 11 | LEU | LYS | TRP | ALA | PHE | SER | MET | TYR | ASP | LEU | ||||
| 12 | ASP | GLY | ASN | GLY | TYR | ILE | SER | ARG | SER | GLU | ||||
| 13 | MET | LEU | GLU | ILE | VAL | GLN | ALA | ILE | TYR | LYS | ||||
| 14 | MET | VAL | SER | SER | VAL | MET | LYS | MET | PRO | GLU | ||||
| 15 | ASP | GLU | SER | THR | PRO | GLU | LYS | ARG | THR | ASP | ||||
| 16 | LYS | ILE | PHE | ARG | GLN | MET | ASP | THR | ASN | ASN | ||||
| 17 | ASP | GLY | LYS | LEU | SER | LEU | GLU | GLU | PHE | ILE | ||||
| 18 | ARG | GLY | ALA | LYS | SER | ASP | PRO | SER | ILE | VAL | ||||
| 19 | ARG | LEU | LEU | GLN | CYS | ASP | PRO | SER | SER | ALA | ||||
| 20 | SER | GLN | PHE |
Samples:
sample_1: Myristoylated VILIP-3, [U-100% 15N], 0.5 – 1 mM; H2O 90%; D2O 10%
sample_2: Myristoylated VILIP-3, [U-100% 13C; U-100% 15N], 0.5-1 mM; H2O 90%; D2O 10%
sample_3: Myristoylated VILIP-3, [U-100% 13C; U-100% 15N], 0.5-1 mM; D2O 100%
sample_4: Myristoylated VILIP-3 0.5-1 mM; D2O 100%
sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts