BMRB Entry 4564
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4564
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Title: NMR Structure of the Bovine Prion Protein
Deposition date: 1999-12-15 Original release date: 2000-05-24
Authors: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt
Citation: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt. "NMR Structure of the Bovine Prion Protein" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
bovine prion protein, polymer, 264 residues, 13137 Da.
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
bovine prion protein: MVKSHIGSWILVLFVAMWSD
VGLSKKRPKPGGGWNTGGSR
YPGQGSPGGNRYPPQGGGGW
GQPHGGGWGQPHGGGWGQPH
GGGWGQPHGGGWGQPHGGGG
WGQGGTHGQWNKPSKPKTNM
KHVAGAAAAGAVVGGLGGYM
LGSAMSRPLIHFGSDYEDRY
YRENMHRYPNQVYYRPVDQY
SNQNNFVHDCVNITVKEHTV
TTTTKGENFTETDIKMMERV
VEQMCITQYQRESQAYYQRG
ASVILFSSPPVILLISFLIF
LIVG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 483 |
| 15N chemical shifts | 226 |
| 1H chemical shifts | 1199 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bPrP(23-230) | 1 |
Entities:
Entity 1, bPrP(23-230) 264 residues - 13137 Da.
| 1 | MET | VAL | LYS | SER | HIS | ILE | GLY | SER | TRP | ILE | ||||
| 2 | LEU | VAL | LEU | PHE | VAL | ALA | MET | TRP | SER | ASP | ||||
| 3 | VAL | GLY | LEU | SER | LYS | LYS | ARG | PRO | LYS | PRO | ||||
| 4 | GLY | GLY | GLY | TRP | ASN | THR | GLY | GLY | SER | ARG | ||||
| 5 | TYR | PRO | GLY | GLN | GLY | SER | PRO | GLY | GLY | ASN | ||||
| 6 | ARG | TYR | PRO | PRO | GLN | GLY | GLY | GLY | GLY | TRP | ||||
| 7 | GLY | GLN | PRO | HIS | GLY | GLY | GLY | TRP | GLY | GLN | ||||
| 8 | PRO | HIS | GLY | GLY | GLY | TRP | GLY | GLN | PRO | HIS | ||||
| 9 | GLY | GLY | GLY | TRP | GLY | GLN | PRO | HIS | GLY | GLY | ||||
| 10 | GLY | TRP | GLY | GLN | PRO | HIS | GLY | GLY | GLY | GLY | ||||
| 11 | TRP | GLY | GLN | GLY | GLY | THR | HIS | GLY | GLN | TRP | ||||
| 12 | ASN | LYS | PRO | SER | LYS | PRO | LYS | THR | ASN | MET | ||||
| 13 | LYS | HIS | VAL | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ||||
| 14 | ALA | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | MET | ||||
| 15 | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ILE | ||||
| 16 | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | TYR | ||||
| 17 | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ASN | ||||
| 18 | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | TYR | ||||
| 19 | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | CYS | ||||
| 20 | VAL | ASN | ILE | THR | VAL | LYS | GLU | HIS | THR | VAL | ||||
| 21 | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | THR | ||||
| 22 | GLU | THR | ASP | ILE | LYS | MET | MET | GLU | ARG | VAL | ||||
| 23 | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | GLN | ||||
| 24 | ARG | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | GLY | ||||
| 25 | ALA | SER | VAL | ILE | LEU | PHE | SER | SER | PRO | PRO | ||||
| 26 | VAL | ILE | LEU | LEU | ILE | SER | PHE | LEU | ILE | PHE | ||||
| 27 | LEU | ILE | VAL | GLY |
Samples:
sample_1: bovine prion protein 1.0 mM
sample_2: bovine prion protein, [U-15N], 1.0 mM
sample_3: bovine prion protein, [U-15N; U-13C], 1.0 mM
sample_conditions: pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H-15N NOESY | not available | not available | not available |
| 1H-1H-13C NOESY | not available | not available | not available |
| HCCH COSY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(COCA)NNH | not available | not available | not available |
| ct-HNCA | not available | not available | not available |
Software:
PROSA -
XEASY -
DYANA -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAA01467 BAA01468 BAA19253 BAE78583 BAE78586 |
| EMBL | CAA39368 CAA52781 CAC37367 |
| GB | AAB25514 AAC43030 AAD13290 AAD19984 AAD19998 |
| REF | NP_001258555 NP_001291815 NP_851358 XP_005906596 XP_006039785 |
| SP | B5SY89 P10279 P40242 Q5UAF1 Q5UJG1 |
| TPG | DAA23426 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts