BMRB Entry 4578
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4578
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Title: 1H, 13C and 15N NMR Assignment of the Hyperstable Quintuple Mutant of Pseudomonas aeruginosa Cytochrome c-551. PubMed: 10918067
Deposition date: 2000-01-28 Original release date: 2000-11-29
Authors: Hasegawa, Jun; Sambongi, Yoshihiro; Kobayashi, Yuji; Igarashi, Yasuo
Citation: Hasegawa, Jun; Uchiyama, Susumu; Yuko, Tanimoto; Masayuki, Mizutani; Yuji, Kobayashi; Yoshihiro, Sambongi; Igarashi, Yasuo. "Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart" J. Biol. Chem. 275, 37824-37828 (2000).
Assembly members:
Psedomonas aeruginosa cytochrome c-551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Psedomonas aeruginosa cytochrome c-551: EDPEVLAKNKGCMACHAIDT
KMVGPAYKDVAAKYAGQAGA
EAYLAQRIKNGSQGVWGPIP
MPPNAVSDDEAQTLAKWILS
QK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 351 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 531 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cyt c551 | 1 |
| 2 | heme c | 2 |
Entities:
Entity 1, Cyt c551 82 residues - Formula weight is not available
| 1 | GLU | ASP | PRO | GLU | VAL | LEU | ALA | LYS | ASN | LYS | ||||
| 2 | GLY | CYS | MET | ALA | CYS | HIS | ALA | ILE | ASP | THR | ||||
| 3 | LYS | MET | VAL | GLY | PRO | ALA | TYR | LYS | ASP | VAL | ||||
| 4 | ALA | ALA | LYS | TYR | ALA | GLY | GLN | ALA | GLY | ALA | ||||
| 5 | GLU | ALA | TYR | LEU | ALA | GLN | ARG | ILE | LYS | ASN | ||||
| 6 | GLY | SER | GLN | GLY | VAL | TRP | GLY | PRO | ILE | PRO | ||||
| 7 | MET | PRO | PRO | ASN | ALA | VAL | SER | ASP | ASP | GLU | ||||
| 8 | ALA | GLN | THR | LEU | ALA | LYS | TRP | ILE | LEU | SER | ||||
| 9 | GLN | LYS |
Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: Psedomonas aeruginosa cytochrome c-551, [U-95% 13C; U-95% 15N], 1.0 mM
Ex-cond_1: pH: 5.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | Ex-cond_1 |
| 2D DQF-COSY | sample_1 | not available | Ex-cond_1 |
| 2D 1H-15N HSQC | sample_1 | not available | Ex-cond_1 |
| 2D 1H-13C CT-HSQC | sample_1 | not available | Ex-cond_1 |
| 3D 15N edited NOESY-HSQC | sample_1 | not available | Ex-cond_1 |
| 3D HNHA | sample_1 | not available | Ex-cond_1 |
| 3D HNHB | sample_1 | not available | Ex-cond_1 |
| 3D HNCACB | sample_1 | not available | Ex-cond_1 |
| 3D CBCA(CO)NNH | sample_1 | not available | Ex-cond_1 |
| 3D (HB)CBCACO(CA)HA | sample_1 | not available | Ex-cond_1 |
| 3D HNCO | sample_1 | not available | Ex-cond_1 |
| 3D HCCH-TOCSY | sample_1 | not available | Ex-cond_1 |
| 3D 13C,15N edited NOESY-HSQC | sample_1 | not available | Ex-cond_1 |
Software:
PIPP -
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
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