BMRB Entry 5220
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5220
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Title: Solution Structure and dynamics of melanoma inhibitory activity protein
Deposition date: 2001-11-29 Original release date: 2002-08-23
Authors: Lougheed, J.; Domaille, P.; Handel, T.
Citation: Lougheed, J.; Domaille, P.; Handel, T.. "Solution Structure and Dynamics of Melanoma Inhibitory Activity Protein" J. Biomol. NMR 22, 211-223 (2002).
Assembly members:
melanoma inhibitory activity protein, polymer, 108 residues, 12237 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
melanoma inhibitory activity protein: MGPMPKLADRKLCADQECSH
PISMAVALQDYMAPDCRFLT
IHRGQVVYVFSKLKGRGRLF
WGGSVQGDYYGDLAARLGYF
PSSIVREDQTLKPGKVDVKT
DKWDFYCQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 386 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 764 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Melanoma Inhibitory Activity Protein | 1 |
Entities:
Entity 1, Melanoma Inhibitory Activity Protein 108 residues - 12237 Da.
| 1 | MET | GLY | PRO | MET | PRO | LYS | LEU | ALA | ASP | ARG | ||||
| 2 | LYS | LEU | CYS | ALA | ASP | GLN | GLU | CYS | SER | HIS | ||||
| 3 | PRO | ILE | SER | MET | ALA | VAL | ALA | LEU | GLN | ASP | ||||
| 4 | TYR | MET | ALA | PRO | ASP | CYS | ARG | PHE | LEU | THR | ||||
| 5 | ILE | HIS | ARG | GLY | GLN | VAL | VAL | TYR | VAL | PHE | ||||
| 6 | SER | LYS | LEU | LYS | GLY | ARG | GLY | ARG | LEU | PHE | ||||
| 7 | TRP | GLY | GLY | SER | VAL | GLN | GLY | ASP | TYR | TYR | ||||
| 8 | GLY | ASP | LEU | ALA | ALA | ARG | LEU | GLY | TYR | PHE | ||||
| 9 | PRO | SER | SER | ILE | VAL | ARG | GLU | ASP | GLN | THR | ||||
| 10 | LEU | LYS | PRO | GLY | LYS | VAL | ASP | VAL | LYS | THR | ||||
| 11 | ASP | LYS | TRP | ASP | PHE | TYR | CYS | GLN |
Samples:
sample_1: melanoma inhibitory activity protein, [U-13C; U-15N], 0.7 – 0.9 mM; sodium acetate, [U-2H], 20 mM; azide 0.02%
sample_cond_1: ionic strength: 20 mM; pH: 4.33; pressure: 1 atm; temperature: 298 K
sample_cond_2: ionic strength: 20 mM; pH*: 4.33; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCANNH | not available | not available | not available |
| 3D CBCA(CO)NNH | not available | not available | not available |
| 4D HCC(CO)NNH | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 2D CBHD | not available | not available | not available |
| 2D CBHE | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| 4D 13C/15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 4D 13C-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY(centered on aromatic carbons) | not available | not available | not available |
| 3D 1H-TOCSY-relayed ct-[13C, 1H]-HMQC | not available | not available | not available |
Software:
X-PLOR v3.851 - refinement, structure solution
ARIA - automated assignment of NOEs, structure calculation
ANSIG v3.3 - data analysis
AZARA v2.0 - processing
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 4731 |
| PDB | |
| DBJ | BAG63518 |
| EMBL | CAA53203 CAA59195 CAG46449 |
| GenBank | AAF76220 AAH05910 AAP35693 AAP36443 AAX29533 |
| REF | NP_006524 XP_001098600 XP_001098701 XP_001146858 XP_001147008 |
| SWISS-PROT | Q16674 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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or all simulated shifts