BMRB Entry 5493
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5493
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Title: Letter to the Editor: Backbone 1H, 15N and 13C resonance assignments of the Staphylococcus aureus acyl carrier protein synthase (AcpS)
Deposition date: 2002-08-07 Original release date: 2002-12-23
Authors: Liu, Dingjiang; Black, Todd; Macinga, David; Palermo, Robert; Wyss, Daniel
Citation: Liu, Dingjiang; Black, Todd; Macinga, David; Palermo, Robert; Wyss, Daniel. "Letter to the Editor: Backbone 1H, 15N and 13C resonance assignments of the Staphylococcus aureus acyl carrier protein synthase (AcpS)" J. Biomol. NMR 24, 273-274 (2002).
Assembly members:
ACP synthase, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Eubacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ACP synthase: MIHGIGVDLIEIDRIQALYS
KQPKLVERILTKNEQHKFNN
FTHEQRKIEFLAGRFATKEA
FSKALGTGLGKHVAFNDIDC
YNDELGKPKIDYEGFIVHVS
ISHTEHYAMSQVVLEKSAF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 312 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AcpS, unit I | 1 |
| 2 | AcpS, unit II | 1 |
| 3 | AcpS, unit III | 1 |
Entities:
Entity 1, AcpS, unit I 119 residues - Formula weight is not available
| 1 | MET | ILE | HIS | GLY | ILE | GLY | VAL | ASP | LEU | ILE | ||||
| 2 | GLU | ILE | ASP | ARG | ILE | GLN | ALA | LEU | TYR | SER | ||||
| 3 | LYS | GLN | PRO | LYS | LEU | VAL | GLU | ARG | ILE | LEU | ||||
| 4 | THR | LYS | ASN | GLU | GLN | HIS | LYS | PHE | ASN | ASN | ||||
| 5 | PHE | THR | HIS | GLU | GLN | ARG | LYS | ILE | GLU | PHE | ||||
| 6 | LEU | ALA | GLY | ARG | PHE | ALA | THR | LYS | GLU | ALA | ||||
| 7 | PHE | SER | LYS | ALA | LEU | GLY | THR | GLY | LEU | GLY | ||||
| 8 | LYS | HIS | VAL | ALA | PHE | ASN | ASP | ILE | ASP | CYS | ||||
| 9 | TYR | ASN | ASP | GLU | LEU | GLY | LYS | PRO | LYS | ILE | ||||
| 10 | ASP | TYR | GLU | GLY | PHE | ILE | VAL | HIS | VAL | SER | ||||
| 11 | ILE | SER | HIS | THR | GLU | HIS | TYR | ALA | MET | SER | ||||
| 12 | GLN | VAL | VAL | LEU | GLU | LYS | SER | ALA | PHE |
Samples:
sample_1: ACP synthase, [U-13C; U-15N; U-2H], 1.0 mM; KiPO4 0.075 M
condition_1: ionic strength: 0.45 M; pH: 7.5 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
Software:
FELIX v2000 - data processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB43158 BAB58233 BAB95860 BAF68248 BAF78939 |
| EMBL | CAA76220 CAG41140 CAG43783 CAI81645 CAQ50498 |
| GB | AAW37023 ABD20944 ABD31340 ABQ49890 ABR52975 |
| REF | NP_372595 NP_375179 NP_646812 WP_000581193 WP_000581194 |
| SP | A5IUL7 A6QIR6 A6U3F7 A7X4P8 A8Z4X4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts