BMRB Entry 6249
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6249
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for BD37
Deposition date: 2004-06-23 Original release date: 2005-02-08
Authors: Yang, Yin-Shan; Delbecq, Stephane; Roumestand, Christian
Citation: Yang, Yin-Shan; Delbecq, Stephane; Strub, Marie-Paule; Lohr, Frank; Schetters, Theo; Gorenflot, Andre; Precigout, Eric; Roumestand, Christian. "Letter to the Editor: 1H, 15N and 13C Backbone resonance assignments of the 37 kDA surface antigen protein Bd37 from Babesia divergens." J. Biomol. NMR 31, 67-68 (2005).
Assembly members:
BD37, polymer, 294 residues, 37000 Da.
Natural source: Common Name: Babesia divergens Taxonomy ID: 32595 Superkingdom: Eukaryota Kingdom: not available Genus/species: Babesia divergens
Experimental source: Production method: recombinant technology
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 557 |
| 15N chemical shifts | 278 |
| 13C chemical shifts | 829 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BD37 | 1 |
Entities:
Entity 1, BD37 294 residues - 37000 Da.
| 1 | CYS | THR | ASN | LEU | ASN | GLY | SER | GLN | GLU | PRO | ||||
| 2 | ALA | ALA | ALA | ASN | PRO | VAL | VAL | SER | THR | PRO | ||||
| 3 | GLY | ASN | ASP | ALA | GLN | GLN | ALA | GLY | THR | GLN | ||||
| 4 | GLN | GLY | GLY | ALA | ASN | SER | LYS | SER | VAL | PRO | ||||
| 5 | GLU | GLN | GLN | PRO | GLN | GLN | ALA | ALA | GLY | GLU | ||||
| 6 | THR | THR | ALA | THR | VAL | VAL | VAL | LYS | THR | LEU | ||||
| 7 | ASP | VAL | LEU | ARG | GLY | GLU | LEU | ARG | GLY | GLN | ||||
| 8 | ARG | GLU | ALA | PHE | LEU | SER | GLU | ILE | ILE | LYS | ||||
| 9 | SER | ASP | GLY | PRO | PHE | THR | ILE | LEU | GLN | LEU | ||||
| 10 | VAL | GLY | TYR | LEU | ARG | VAL | VAL | ASP | THR | ASP | ||||
| 11 | LEU | LEU | LEU | LYS | VAL | ASP | SER | THR | LYS | VAL | ||||
| 12 | ASP | GLU | ALA | GLY | LYS | LYS | VAL | LYS | ALA | TYR | ||||
| 13 | LEU | GLU | LYS | ILE | GLY | ILE | ARG | GLY | ASP | SER | ||||
| 14 | VAL | GLU | ALA | ALA | LEU | ASP | ASN | LEU | MET | ILE | ||||
| 15 | LYS | VAL | TYR | GLU | ILE | THR | LYS | GLY | THR | VAL | ||||
| 16 | GLU | SER | SER | ALA | GLN | GLY | THR | ASP | SER | GLU | ||||
| 17 | GLU | LEU | LYS | THR | LEU | LEU | LEU | LYS | PHE | SER | ||||
| 18 | GLU | ASP | LEU | LYS | ALA | GLU | GLN | GLU | LEU | HIS | ||||
| 19 | SER | GLU | ALA | LYS | GLY | GLY | GLU | ALA | LEU | LEU | ||||
| 20 | SER | SER | MET | LYS | THR | GLN | HIS | ASP | GLU | LEU | ||||
| 21 | LEU | LYS | LYS | PHE | ALA | ALA | LEU | THR | PRO | THR | ||||
| 22 | PHE | LEU | THR | SER | GLU | ASP | ILE | SER | GLY | TYR | ||||
| 23 | LEU | THR | VAL | PRO | GLU | TYR | GLY | ALA | PRO | MET | ||||
| 24 | ASN | ALA | ALA | LYS | TRP | LYS | LYS | VAL | GLU | GLY | ||||
| 25 | MET | ILE | HIS | GLY | LYS | LEU | GLU | SER | SER | GLU | ||||
| 26 | VAL | PRO | ALA | ASN | LEU | LYS | ALA | LEU | VAL | ALA | ||||
| 27 | GLU | LEU | ILE | GLU | LEU | ARG | GLU | GLN | MET | MET | ||||
| 28 | ASP | LEU | LEU | TYR | GLY | PRO | ILE | GLY | HIS | HIS | ||||
| 29 | ASP | CYS | ALA | ALA | GLY | SER | GLY | GLN | GLY | SER | ||||
| 30 | SER | LYS | LEU | ASN |
Samples:
sample_1: BD37, [U-95% 13C; U90% 15N; U80% 2H], 0.8 – 0.1 mM; Sodium Phosphate10 – 1 mM; Sodium Chloride50 – 5 mM
Ex-cond_1: pH: 6.8; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | Ex-cond_1 |
Software:
GIFA v4 -
NMR spectrometers:
- BRUKER AVANCE 600 MHz
- BRUKER AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts