BMRB Entry 6374
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6374
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Title: NMR STRUCTURE OF THE FIRST PHD FINGER OF AUTOIMMUNE REGULATOR PROTEIN (AIRE1): INSIGHTS INTO APECED PubMed: 15649886
Deposition date: 2004-11-01 Original release date: 2005-02-10
Authors: Bottomley, Matthew; Stier, Gunter; Krasotkina, Julya; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna
Citation: Bottomley, Matthew; Stier, Gunter; Pennacchini, D.; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna. "NMR structure of the first PHD finger of autoimmune regulator protein (AIRE1). Insights into autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) disease" J. Biol. Chem. 280, 11505-11512 (2005).
Assembly members:
first PHD finger of AIRE1, polymer, 66 residues, 7138 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
first PHD finger of AIRE1: GAMAQKNEDECAVCRDGGEL
ICCDGCPRAFHLACLSPPLR
EIPSGTWRCSSCLQATVQEV
QPRAEE
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 201 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 649 |
| residual dipolar couplings | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AIRE1-PHD1 monomer | 1 |
| 2 | ZINC (II) ION, 1 | 2 |
| 3 | ZINC (II) ION, 2 | 2 |
Entities:
Entity 1, AIRE1-PHD1 monomer 66 residues - 7138 Da.
| 1 | GLY | ALA | MET | ALA | GLN | LYS | ASN | GLU | ASP | GLU | ||||
| 2 | CYS | ALA | VAL | CYS | ARG | ASP | GLY | GLY | GLU | LEU | ||||
| 3 | ILE | CYS | CYS | ASP | GLY | CYS | PRO | ARG | ALA | PHE | ||||
| 4 | HIS | LEU | ALA | CYS | LEU | SER | PRO | PRO | LEU | ARG | ||||
| 5 | GLU | ILE | PRO | SER | GLY | THR | TRP | ARG | CYS | SER | ||||
| 6 | SER | CYS | LEU | GLN | ALA | THR | VAL | GLN | GLU | VAL | ||||
| 7 | GLN | PRO | ARG | ALA | GLU | GLU |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: first PHD finger of AIRE1, [U-13C; U-15N], 1 mM; NaCl 150 mM; phosphate 20 mM; DTT 5 mM
Ex-cond_1: ionic strength: 0.15 M; pH*: 6.3; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | not available | Ex-cond_1 |
| CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
| CBCANH | sample_1 | not available | Ex-cond_1 |
| 3D HCCONH-TOCSY | sample_1 | not available | Ex-cond_1 |
| CCONH-TOCSY | sample_1 | not available | Ex-cond_1 |
| HCCH-TOCSY | sample_1 | not available | Ex-cond_1 |
| 2D 1H-15N HMQC | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB |