BMRB Entry 6419

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6419

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2 PubMed: 15557330

Deposition date: 2004-12-10 Original release date: 2005-04-26

Authors: Chattopadhyay, A.; Jones, N.; Nietlispach, D.; Nielsen, P.; Voorheis, H.; Mott, H.; Carrington, M.

Citation: Chattopadhyay, A.; Jones, N.; Nietlispach, D.; Nielsen, P.; Voorheis, H.; Mott, H.; Carrington, M.. "Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2" J. Biol. Chem. 280, 7228-7235 (2005).

Assembly members:
Variant surface glycoprotein MITAT 1.2, polymer, 80 residues, Formula weight is not available

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Variant surface glycoprotein MITAT 1.2: GSHMLEVLTQKHKPAESQQQ AAETEGSCNKKDQNECKSPC KWHNDAENKKCTLDKEEAKK VADETAKDGKTGNTNTTGSS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	231
15N chemical shifts	79
1H chemical shifts	433

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Variant surface glycoprotein MITAT 1.2	1

Entities:

Entity 1, Variant surface glycoprotein MITAT 1.2 80 residues - Formula weight is not available

1	GLY	SER	HIS	MET	LEU	GLU	VAL	LEU	THR	GLN
2	LYS	HIS	LYS	PRO	ALA	GLU	SER	GLN	GLN	GLN
3	ALA	ALA	GLU	THR	GLU	GLY	SER	CYS	ASN	LYS
4	LYS	ASP	GLN	ASN	GLU	CYS	LYS	SER	PRO	CYS
5	LYS	TRP	HIS	ASN	ASP	ALA	GLU	ASN	LYS	LYS
6	CYS	THR	LEU	ASP	LYS	GLU	GLU	ALA	LYS	LYS
7	VAL	ALA	ASP	GLU	THR	ALA	LYS	ASP	GLY	LYS
8	THR	GLY	ASN	THR	ASN	THR	THR	GLY	SER	SER

Samples:

sample_1: Variant surface glycoprotein MITAT 1.2, [U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_2: Variant surface glycoprotein MITAT 1.2, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-SEPARATED NOESY	not available	not available	not available
3D 13C-SEPARATED NOESY	not available	not available	not available
CLEANEX	not available	not available	not available

Software:

ARIA v1.1 - refinement, structure solution

AZARA v2.7 - structure solution

ANSIG v3.3 - structure solution

CNS v1.1 - structure solution

NMR spectrometers:

BRUKER DRX 600 MHz

PDB	1XU6
EMBL	CAA40081 CAQ57294
SWISS-PROT	P26332

Biological Magnetic Resonance Data Bank