BMRB Entry 6594
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6594
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Title: Elucidation of the high-resolution solution structure of a trypanosomatid FYVE domain
Deposition date: 2005-04-14 Original release date: 2007-11-14
Authors: Mertens, H.; Callaghan, J.; McConville, M.; Gooley, P.
Citation: Mertens, H.; Callaghan, J.; Swarbrick, J.; McConville, M.; Gooley, P.. "A high-resolution solution structure of a trypanosomatid FYVE domain" Protein Sci. 16, 2552-2559 (2007).
Assembly members:
Leishmania major FYVE domain containing protein 1, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Leishmania major Taxonomy ID: 5664 Superkingdom: Eukaryota Kingdom: not available Genus/species: Leishmania major
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Leishmania major FYVE domain containing protein 1: GPLGSMGEKQSKGYWQEDED
APACNGCGCVFTTTVRRHHC
RNCGYVLCGDCSRHRAAIPM
RGITEPERVCDACYLALRSS
NMAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 323 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 492 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LM5-1 | 1 |
| 2 | ZINC (II) ION, 1 | 2 |
| 3 | ZINC (II) ION, 2 | 2 |
Entities:
Entity 1, LM5-1 84 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | MET | GLY | GLU | LYS | GLN | ||||
| 2 | SER | LYS | GLY | TYR | TRP | GLN | GLU | ASP | GLU | ASP | ||||
| 3 | ALA | PRO | ALA | CYS | ASN | GLY | CYS | GLY | CYS | VAL | ||||
| 4 | PHE | THR | THR | THR | VAL | ARG | ARG | HIS | HIS | CYS | ||||
| 5 | ARG | ASN | CYS | GLY | TYR | VAL | LEU | CYS | GLY | ASP | ||||
| 6 | CYS | SER | ARG | HIS | ARG | ALA | ALA | ILE | PRO | MET | ||||
| 7 | ARG | GLY | ILE | THR | GLU | PRO | GLU | ARG | VAL | CYS | ||||
| 8 | ASP | ALA | CYS | TYR | LEU | ALA | LEU | ARG | SER | SER | ||||
| 9 | ASN | MET | ALA | GLY |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Leishmania major FYVE domain containing protein 1, [U-15N; U-13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; H2O 90%; D2O 10%
sample_2: Leishmania major FYVE domain containing protein 1, [U-15N; U-13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; D2O 99.99%
sample_3: Leishmania major FYVE domain containing protein 1, [U-10% 13C], 0.8 mM; sodium phosphate buffer 50 mM; KCl 50 mM; DTT 1 mM; AEBSF 1 mM; sodium azide 0.05%; H2O 90%; D2O 10%
sample_cond_1: pH: 7.4; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 2D 13C-ct-HSQC | not available | not available | sample_cond_1 |
Software:
VNMR v6.1c - collection
NMRPipe v2.2 - processing
XEASY v1.3 - data analysis
CYANA v1.0.7 - structure solution
X-PLOR NIH v2.9.1 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
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