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BMRB Entry 6759

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6759
MolProbity Validation Chart

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Title: NEAT NMR Resonance Assignments   PubMed: 16258836

Deposition date: 2005-08-03 Original release date: 2005-11-07

Authors: Pilpa, Rosemarie; Clubb, Robert

Citation: Pilpa, Rosemarie; Clubb, Robert. "NMR Resonance Assignments of the NEAT (NEAr Transporter) Domain from the Staphylococcus aureus IsdH Protein"  J. Biomol. NMR 33, 137-137 (2005).

Assembly members:
NEAT domain, polymer, 145 residues, Formula weight is not available

Natural source:   Common Name: polypeptide   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NEAT domain: MADESLKDAIKDPALENKEH DIGPREQVNFQLLDKNNETQ YYHFFSIKDPADVYYTKKKA EVELDINTASTWKKFEVYEN NQKLPVRLVSYSPVPEDHAY IRFPVSDGTQELKIVSSTQI DDGEETNYDYTKLVFAKPIY NDPSL

Data sets:
Data typeCount
13C chemical shifts565
15N chemical shifts123
1H chemical shifts820

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NEAT domain1

Entities:

Entity 1, NEAT domain 145 residues - Formula weight is not available

1   METALAASPGLUSERLEULYSASPALAILE
2   LYSASPPROALALEUGLUASNLYSGLUHIS
3   ASPILEGLYPROARGGLUGLNVALASNPHE
4   GLNLEULEUASPLYSASNASNGLUTHRGLN
5   TYRTYRHISPHEPHESERILELYSASPPRO
6   ALAASPVALTYRTYRTHRLYSLYSLYSALA
7   GLUVALGLULEUASPILEASNTHRALASER
8   THRTRPLYSLYSPHEGLUVALTYRGLUASN
9   ASNGLNLYSLEUPROVALARGLEUVALSER
10   TYRSERPROVALPROGLUASPHISALATYR
11   ILEARGPHEPROVALSERASPGLYTHRGLN
12   GLULEULYSILEVALSERSERTHRGLNILE
13   ASPASPGLYGLUGLUTHRASNTYRASPTYR
14   THRLYSLEUVALPHEALALYSPROILETYR
15   ASNASPPROSERLEU

Samples:

NEAT_domain: NEAT domain, [U-13C; U-15N], 1 mM; sodium phospate 50 mM; NaCl 100 mM; sodium azide 0.01%

NMR_conditions: pH: 6; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCNEAT_domainnot availableNMR_conditions
1H13C_HSQCNEAT_domainnot availableNMR_conditions
15N-edited NOESYNEAT_domainnot availableNMR_conditions
HNHANEAT_domainnot availableNMR_conditions
HNHBNEAT_domainnot availableNMR_conditions
HNCACBNEAT_domainnot availableNMR_conditions
CBCACONHNEAT_domainnot availableNMR_conditions
CCCONHNEAT_domainnot availableNMR_conditions
HNCANEAT_domainnot availableNMR_conditions
HNCONEAT_domainnot availableNMR_conditions
HNCACONEAT_domainnot availableNMR_conditions
HBHACONHNEAT_domainnot availableNMR_conditions
15N-edited TOCSYNEAT_domainnot availableNMR_conditions
13C-edited NOESY in D2ONEAT_domainnot availableNMR_conditions
HCCH-TOCSYNEAT_domainnot availableNMR_conditions
HCCH-COSYNEAT_domainnot availableNMR_conditions
HNHNNEAT_domainnot availableNMR_conditions
13C-edited NOESY in H2ONEAT_domainnot availableNMR_conditions

Software:

NMRView -

PIPP -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts