BMRB Entry 6910
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6910
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Title: 1H,13C,15N chemical shift assignment for SMP-1 (Small Myrisoylated Protein-1) from Leishmania PubMed: 16601858
Deposition date: 2005-11-29 Original release date: 2006-04-14
Authors: Gooley, Paul; Mertens, Haydyn; Tull, Dedreia; McConville, Malcolm
Citation: Gooley, Paul; Mertens, Haydyn; Tull, Dedreia; McConville, Malcolm. "1H, 13C and 15N Resonance Assignments of SMP-1: A Small Myristoylated Protein from Leishmania major" J. Biomol. NMR 36, 26-26 (2006).
Assembly members:
SMP-1, polymer, 131 residues, 14988.8 Da.
Natural source: Common Name: Leishmania major Taxonomy ID: 5664 Superkingdom: Eukaryota Kingdom: Protista Genus/species: Leishmania major
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SMP-1: MGCGASSENSSVTYVNGRPT
FVGEEVTKGFEKDNGLLFRI
VNKKKKQWAYYNDTTQYEMH
VLVTFNEDCDIKALGKTKLE
QQENGEWVASVVVYPCETEM
FIEGRVNGFKSKMDALPLSE
EYRQHQAEKDK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 824 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SMP-1 | 1 |
Entities:
Entity 1, SMP-1 131 residues - 14988.8 Da.
| 1 | MET | GLY | CYS | GLY | ALA | SER | SER | GLU | ASN | SER | ||||
| 2 | SER | VAL | THR | TYR | VAL | ASN | GLY | ARG | PRO | THR | ||||
| 3 | PHE | VAL | GLY | GLU | GLU | VAL | THR | LYS | GLY | PHE | ||||
| 4 | GLU | LYS | ASP | ASN | GLY | LEU | LEU | PHE | ARG | ILE | ||||
| 5 | VAL | ASN | LYS | LYS | LYS | LYS | GLN | TRP | ALA | TYR | ||||
| 6 | TYR | ASN | ASP | THR | THR | GLN | TYR | GLU | MET | HIS | ||||
| 7 | VAL | LEU | VAL | THR | PHE | ASN | GLU | ASP | CYS | ASP | ||||
| 8 | ILE | LYS | ALA | LEU | GLY | LYS | THR | LYS | LEU | GLU | ||||
| 9 | GLN | GLN | GLU | ASN | GLY | GLU | TRP | VAL | ALA | SER | ||||
| 10 | VAL | VAL | VAL | TYR | PRO | CYS | GLU | THR | GLU | MET | ||||
| 11 | PHE | ILE | GLU | GLY | ARG | VAL | ASN | GLY | PHE | LYS | ||||
| 12 | SER | LYS | MET | ASP | ALA | LEU | PRO | LEU | SER | GLU | ||||
| 13 | GLU | TYR | ARG | GLN | HIS | GLN | ALA | GLU | LYS | ASP | ||||
| 14 | LYS |
Samples:
sample_1: SMP-1, [U-13C; U-15N], 1 mM; Dithiothreitol 5 mM; Sodium Phosphate 50 mM; H2O 90%; D2O 10%
conditions_1: ionic strength: 50 mM; pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| H(CCO)NH | sample_1 | not available | conditions_1 |
| C(CO)NH | sample_1 | not available | conditions_1 |
| HBHA(CO)NH | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| HNHA | sample_1 | not available | conditions_1 |
| HNHB | sample_1 | not available | conditions_1 |
| (HB)CB(CGCD)HD | sample_1 | not available | conditions_1 |
| 1H-TOCSY-relayed ct-(13C,1H)HMQC | sample_1 | not available | conditions_1 |
| 15N-HSQC | sample_1 | not available | conditions_1 |
| 13C-HSQC | sample_1 | not available | conditions_1 |
Software:
NMRPipe v2.3, Frank Delaglio -
NMRView v5.2.2, Bruce Johnson -
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAJ04271 CAM67513 CBZ33770 |
| GB | AAV59017 |
| REF | XP_001465265 XP_001682883 XP_003860476 |
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