BMRB Entry 10132
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10132
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Title: 1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551 PubMed: 17658890
Deposition date: 2007-04-15 Original release date: 2008-02-11
Authors: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki
Citation: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential." Biochemistry 46, 9215-9224 (2007).
Assembly members:
cytochrome c551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
cytochrome c551: EDPEVLFKNKGCVACHAIDT
KMVGPAYKDVAAKFAGQAGA
EAELAQRIKNGSQGVWGPIP
MPPNAVSDDEAQTLAKWVLS
QK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, cytochrome c 82 residues - Formula weight is not available
| 1 | GLU | ASP | PRO | GLU | VAL | LEU | PHE | LYS | ASN | LYS | ||||
| 2 | GLY | CYS | VAL | ALA | CYS | HIS | ALA | ILE | ASP | THR | ||||
| 3 | LYS | MET | VAL | GLY | PRO | ALA | TYR | LYS | ASP | VAL | ||||
| 4 | ALA | ALA | LYS | PHE | ALA | GLY | GLN | ALA | GLY | ALA | ||||
| 5 | GLU | ALA | GLU | LEU | ALA | GLN | ARG | ILE | LYS | ASN | ||||
| 6 | GLY | SER | GLN | GLY | VAL | TRP | GLY | PRO | ILE | PRO | ||||
| 7 | MET | PRO | PRO | ASN | ALA | VAL | SER | ASP | ASP | GLU | ||||
| 8 | ALA | GLN | THR | LEU | ALA | LYS | TRP | VAL | LEU | SER | ||||
| 9 | GLN | LYS |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: cytochrome c551, [U-15N], 1 mM; potassium phosphate 20 mM; potassium ferricyanide 10 mM
Cond_1: pH: 6.0; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | Cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 10133 1333 1334 1734 1866 2968 |
| PDB | |
| DBJ | BAD11780 BAK92489 BAP19663 BAP48532 BAQ37290 |
| EMBL | CAA35703 CAA35958 CAW25242 CCQ85614 CDH68848 |
| GB | AAG03907 AAT49429 ABJ15480 ABR83995 AEO73006 |
| PRF | 630481A |
| REF | NP_249209 WP_003084872 WP_003118863 WP_003129143 WP_012074083 |
| SP | P00099 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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