BMRB Entry 10306
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10306
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Title: Solution structure of the 1st zf-C2H2 domain from Human B-cell lymphoma 6 protein
Deposition date: 2009-03-10 Original release date: 2010-03-11
Authors: Tomizawa, T.; Sato, M.; Saito, K.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Sato, M.; Saito, K.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 1st zf-C2H2 domain from Human B-cell lymphoma 6 protein" . ., .-..
Assembly members:
zf-C2H2 domain, polymer, 43 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell-free synthesis
Entity Sequences (FASTA):
zf-C2H2 domain: GSSGSSGNGAFFCNECDCRF
SEEASLKRHTLQTHSDKSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 148 |
| 15N chemical shifts | 35 |
| 1H chemical shifts | 214 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-C2H2 domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, zf-C2H2 domain 43 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | GLY | ALA | ||||
| 2 | PHE | PHE | CYS | ASN | GLU | CYS | ASP | CYS | ARG | PHE | ||||
| 3 | SER | GLU | GLU | ALA | SER | LEU | LYS | ARG | HIS | THR | ||||
| 4 | LEU | GLN | THR | HIS | SER | ASP | LYS | SER | GLY | PRO | ||||
| 5 | SER | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-C2H2 domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9825, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
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