BMRB Entry 11144
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11144
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Title: Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein
Deposition date: 2010-03-31 Original release date: 2011-04-01
Authors: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein" . ., .-..
Assembly members:
DnaJ domain, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
DnaJ domain: GSSGSSGSQGDCSYSRTALY
DLLGVPSTATQAQIKAAYYR
QCFLYHPDRNSGSAEAAERF
TRISQAYVVLGSATLRRKYD
RGLLSDEDLRGPGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 390 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 586 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DnaJ domain | 1 |
Entities:
Entity 1, DnaJ domain 99 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | GLN | GLY | ||||
| 2 | ASP | CYS | SER | TYR | SER | ARG | THR | ALA | LEU | TYR | ||||
| 3 | ASP | LEU | LEU | GLY | VAL | PRO | SER | THR | ALA | THR | ||||
| 4 | GLN | ALA | GLN | ILE | LYS | ALA | ALA | TYR | TYR | ARG | ||||
| 5 | GLN | CYS | PHE | LEU | TYR | HIS | PRO | ASP | ARG | ASN | ||||
| 6 | SER | GLY | SER | ALA | GLU | ALA | ALA | GLU | ARG | PHE | ||||
| 7 | THR | ARG | ILE | SER | GLN | ALA | TYR | VAL | VAL | LEU | ||||
| 8 | GLY | SER | ALA | THR | LEU | ARG | ARG | LYS | TYR | ASP | ||||
| 9 | ARG | GLY | LEU | LEU | SER | ASP | GLU | ASP | LEU | ARG | ||||
| 10 | GLY | PRO | GLY | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Gal-binding lectin, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20060524, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9825, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB71671 |
| GB | AAM62307 AAS07471 EAW69658 |
| REF | NP_001181473 NP_115693 XP_001145797 XP_002817906 XP_003815619 |
| SP | Q96LL9 |
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