BMRB Entry 15007
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15007
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Title: Structural Studies on Plasmodium vivax Merozoite Surface Protein-1 PubMed: 17343930
Deposition date: 2006-10-30 Original release date: 2008-02-04
Authors: Babon, Jeffrey; Morgan, William; Geoff, Kelly; John, Eccleston; James, Feeney; Anthony, Holder
Citation: Babon, Jeffrey; Morgan, William; Kelly, Geoff; Eccleston, John; Feeney, James; Holder, Anthony. "Structural studies on Plasmodium vivax merozoite surface protein-1." Mol. Biochem. Parasitol. 153, 31-40 (2007).
Assembly members:
msp-1, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: PLASMODIUM VIVAX Taxonomy ID: 5855 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium vivax
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
msp-1: TMSSEHTCIDTNVPDNAACY
RYLDGTEEWRCLLTFKEEGG
KCVPASNVTCKDNNGGCAPE
AECKMTDSNKIVCKCTKEGS
EPLFEGVFCS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 301 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 487 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | msp-1 | 1 |
Entities:
Entity 1, msp-1 90 residues - Formula weight is not available
| 1 | THR | MET | SER | SER | GLU | HIS | THR | CYS | ILE | ASP | |
| 2 | THR | ASN | VAL | PRO | ASP | ASN | ALA | ALA | CYS | TYR | |
| 3 | ARG | TYR | LEU | ASP | GLY | THR | GLU | GLU | TRP | ARG | |
| 4 | CYS | LEU | LEU | THR | PHE | LYS | GLU | GLU | GLY | GLY | |
| 5 | LYS | CYS | VAL | PRO | ALA | SER | ASN | VAL | THR | CYS | |
| 6 | LYS | ASP | ASN | ASN | GLY | GLY | CYS | ALA | PRO | GLU | |
| 7 | ALA | GLU | CYS | LYS | MET | THR | ASP | SER | ASN | LYS | |
| 8 | ILE | VAL | CYS | LYS | CYS | THR | LYS | GLU | GLY | SER | |
| 9 | GLU | PRO | LEU | PHE | GLU | GLY | VAL | PHE | CYS | SER |
Samples:
sample_1: msp-1, [U-100% 13C; U-100% 15N], 0.5 – 1.0 mM; k-phosphate 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, CD Schwieters, JJ Kuszewski, N Tjandra and GM Clore - structure solution
NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - data analysis
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ41307 BAJ41308 BAJ41309 BAJ41310 BAJ41311 |
| EMBL | CAC82066 CAC82067 CAC82068 CAC82069 CAC82070 |
| GB | AAA29735 AAA63427 AAC37236 AAC37237 AAF91156 |
| REF | XP_001614842 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts