BMRB Entry 15214
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15214
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: MxiHCdelta5 backbone assignment PubMed: 17827155
Deposition date: 2007-04-17 Original release date: 2007-09-12
Authors: Zhang, Lingling
Citation: Zhang, Lingling; Wang, Yu; Olive, Andrew; Smith, Nathan; Picking, William; De Guzman, Roberto; Picking, Wendy. "Identification of the MxiH needle protein residues responsible for anchoring invasion plasmid antigen D to the type III secretion needle tip" J. Biol. Chem. 282, 32144-32151 (2007).
Assembly members:
MxiH, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: Shigella flexneri Taxonomy ID: 623 Superkingdom: Bacteria Kingdom: not available Genus/species: Shigella flexneri
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MxiH: MSVTVPNDDWTLSSLSETFD
DGTQTLQGELTLALDKLAKN
PSNPQLLAEYQSKLSEYTLY
RNAQSNTVKVIKDVDAAILE
HHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 50 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 63 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MxiH | 1 |
Entities:
Entity 1, MxiH 86 residues - Formula weight is not available
| 1 | MET | SER | VAL | THR | VAL | PRO | ASN | ASP | ASP | TRP | ||||
| 2 | THR | LEU | SER | SER | LEU | SER | GLU | THR | PHE | ASP | ||||
| 3 | ASP | GLY | THR | GLN | THR | LEU | GLN | GLY | GLU | LEU | ||||
| 4 | THR | LEU | ALA | LEU | ASP | LYS | LEU | ALA | LYS | ASN | ||||
| 5 | PRO | SER | ASN | PRO | GLN | LEU | LEU | ALA | GLU | TYR | ||||
| 6 | GLN | SER | LYS | LEU | SER | GLU | TYR | THR | LEU | TYR | ||||
| 7 | ARG | ASN | ALA | GLN | SER | ASN | THR | VAL | LYS | VAL | ||||
| 8 | ILE | LYS | ASP | VAL | ASP | ALA | ALA | ILE | LEU | GLU | ||||
| 9 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: MxiH, [U-13C; U-99% 15N], 0.7 mM; NaCl 10 mM; Na Phosphate 10 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts