BMRB Entry 15682
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15682
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii) PubMed: 19636901
Deposition date: 2008-03-14 Original release date: 2008-10-03
Authors: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen
Citation: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1" Biomol. NMR Assignments 2, 187-189 (2008).
Assembly members:
Epidermal_Growth_Factor-like_domain_2, polymer, 61 residues, 6362 Da.
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Epidermal_Growth_Factor-like_domain_2: MANFVQLSETPAACSSNPCG
PEAAGTCKETNSGYICRCNQ
GYRISLDGTGNVTCIVRQES
G
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 218 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 332 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Epidermal Growth Factor-like domain 2 | 1 |
Entities:
Entity 1, Epidermal Growth Factor-like domain 2 61 residues - 6362 Da.
| 1 | MET | ALA | ASN | PHE | VAL | GLN | LEU | SER | GLU | THR | ||||
| 2 | PRO | ALA | ALA | CYS | SER | SER | ASN | PRO | CYS | GLY | ||||
| 3 | PRO | GLU | ALA | ALA | GLY | THR | CYS | LYS | GLU | THR | ||||
| 4 | ASN | SER | GLY | TYR | ILE | CYS | ARG | CYS | ASN | GLN | ||||
| 5 | GLY | TYR | ARG | ILE | SER | LEU | ASP | GLY | THR | GLY | ||||
| 6 | ASN | VAL | THR | CYS | ILE | VAL | ARG | GLN | GLU | SER | ||||
| 7 | GLY |
Samples:
sample_1: Epidermal Growth Factor-like domain 2, [U-98% 13C; U-98% 15N], 0.5 mM; potassium phosphate 25 mM; sodium chloride 50 mM
sample_conditions_1: pH: 6.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - Peak picking, Viewing NMR spectra
NMR spectrometers:
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts