BMRB Entry 15692
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15692
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Title: Backbone assignment of MIC1-CT from Toxoplasma gondii in complex with EGF3 from TgMIC6 PubMed: 19636898
Deposition date: 2008-03-25 Original release date: 2008-10-15
Authors: Saouros, Savvas; Sawmynaden, Kovilen; Simpson, Peter; Matthews, Stephen
Citation: Saouros, Savvas; Sawmynaden, Kovilen; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete resonance assignment of the galectin-like domain of MIC1 from Toxoplasma gondii in complex with the second EGF domain from MIC6 and the backbone assignment in complex with the third EGF domain" Biomol. NMR Assignments 2, 175-177 (2008).
Assembly members:
TgMIC1-CT, polymer, 138 residues, Formula weight is not available
TgMIC6-EGF3, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TgMIC1-CT: MKTEIHGDSTKATLEEGQQL
TLTFISTKLDVAVGSCHSLV
ANFLDGFLKFQTGSNSAFDV
VEVEEPAGPAVLTIGLGHKG
RLAVVLDYTRLNAALGSAAY
VVEDSGCSSSEEVSFQGVGS
GATLVVTTLGESPTAVSA
TgMIC6-EGF3: IVRQESGCEENGCGPPDAVQ
SCRRLTGTAGRLCVCKENFI
ATIDASAHITCKRVPPHYRK
PPFEFGKGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 396 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TgMIC1-CT | 1 |
| 2 | TgMIC6-EGF3 | 2 |
Entities:
Entity 1, TgMIC1-CT 138 residues - Formula weight is not available
| 1 | MET | LYS | THR | GLU | ILE | HIS | GLY | ASP | SER | THR | ||||
| 2 | LYS | ALA | THR | LEU | GLU | GLU | GLY | GLN | GLN | LEU | ||||
| 3 | THR | LEU | THR | PHE | ILE | SER | THR | LYS | LEU | ASP | ||||
| 4 | VAL | ALA | VAL | GLY | SER | CYS | HIS | SER | LEU | VAL | ||||
| 5 | ALA | ASN | PHE | LEU | ASP | GLY | PHE | LEU | LYS | PHE | ||||
| 6 | GLN | THR | GLY | SER | ASN | SER | ALA | PHE | ASP | VAL | ||||
| 7 | VAL | GLU | VAL | GLU | GLU | PRO | ALA | GLY | PRO | ALA | ||||
| 8 | VAL | LEU | THR | ILE | GLY | LEU | GLY | HIS | LYS | GLY | ||||
| 9 | ARG | LEU | ALA | VAL | VAL | LEU | ASP | TYR | THR | ARG | ||||
| 10 | LEU | ASN | ALA | ALA | LEU | GLY | SER | ALA | ALA | TYR | ||||
| 11 | VAL | VAL | GLU | ASP | SER | GLY | CYS | SER | SER | SER | ||||
| 12 | GLU | GLU | VAL | SER | PHE | GLN | GLY | VAL | GLY | SER | ||||
| 13 | GLY | ALA | THR | LEU | VAL | VAL | THR | THR | LEU | GLY | ||||
| 14 | GLU | SER | PRO | THR | ALA | VAL | SER | ALA |
Entity 2, TgMIC6-EGF3 69 residues - Formula weight is not available
| 1 | ILE | VAL | ARG | GLN | GLU | SER | GLY | CYS | GLU | GLU | ||||
| 2 | ASN | GLY | CYS | GLY | PRO | PRO | ASP | ALA | VAL | GLN | ||||
| 3 | SER | CYS | ARG | ARG | LEU | THR | GLY | THR | ALA | GLY | ||||
| 4 | ARG | LEU | CYS | VAL | CYS | LYS | GLU | ASN | PHE | ILE | ||||
| 5 | ALA | THR | ILE | ASP | ALA | SER | ALA | HIS | ILE | THR | ||||
| 6 | CYS | LYS | ARG | VAL | PRO | PRO | HIS | TYR | ARG | LYS | ||||
| 7 | PRO | PRO | PHE | GLU | PHE | GLY | LYS | GLY | GLY |
Samples:
sample_1: TgMIC1-CT, [U-100% 13C; U-100% 15N], 1-1.3 mM; TgMIC6-EGF3, [U-100% 13C; U-100% 15N], 1-1.3 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.9; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAA96466 |
| GB | EPR59679 EPT28103 ESS33997 KFG30856 KFG41657 AAD28185 EPR63801 EPT24678 ESS28844 KFG37976 |
| REF | XP_002368531 XP_002370636 |
| SP | O00834 Q9XYH7 |
| TPE | CEL76086 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts