BMRB Entry 15744
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15744
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Title: Backbone assignment of lipid-free human apolipoprotein E (apoE) PubMed: 19636906
Deposition date: 2008-04-26 Original release date: 2008-10-15
Authors: Zhang, Yonghong; Chen, Jianglei; Wang, Jianjun
Citation: Zhang, Yonghong; Chen, Jianglei; Wang, Jianjun. "A complete backbone spectral assignment of lipid-free human apolipoprotein E (apoE)" Biomol. NMR Assignments 2, 207-210 (2008).
Assembly members:
Apolipoprotein_E, polymer, 308 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Apolipoprotein_E: MHHHHHHHHKVEQAVETEPE
PELRQQTEWQSGQRWELALG
RFWDYLRWVQTLSEQVQEEL
LSSQVTQELRALMDETMKEL
KAYKSELEEQLTPVAEETRA
RLSKELQAAQARLGADMEDV
CGRLVQYRGEVQAMLGQSTE
ELRVRLASHLRKLRKRLLRD
ADDLQKRLAVYQAGAREGAE
RGLSAIRERLGPLVEQGRVR
AATVGSLAGQPLQERAQAWG
ERLRARMEEMGSRTRDRLDE
VKEQVAEVRAKLEEQAQQIR
LQAEAAQARLKSRFEPLAED
MQRQWAGQVEKVQAAEGTSA
APVPSDNH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 579 |
| 15N chemical shifts | 288 |
| 1H chemical shifts | 288 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lipid-free human apolipoprotein E | 1 |
Entities:
Entity 1, Lipid-free human apolipoprotein E 308 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | LYS | ||||
| 2 | VAL | GLU | GLN | ALA | VAL | GLU | THR | GLU | PRO | GLU | ||||
| 3 | PRO | GLU | LEU | ARG | GLN | GLN | THR | GLU | TRP | GLN | ||||
| 4 | SER | GLY | GLN | ARG | TRP | GLU | LEU | ALA | LEU | GLY | ||||
| 5 | ARG | PHE | TRP | ASP | TYR | LEU | ARG | TRP | VAL | GLN | ||||
| 6 | THR | LEU | SER | GLU | GLN | VAL | GLN | GLU | GLU | LEU | ||||
| 7 | LEU | SER | SER | GLN | VAL | THR | GLN | GLU | LEU | ARG | ||||
| 8 | ALA | LEU | MET | ASP | GLU | THR | MET | LYS | GLU | LEU | ||||
| 9 | LYS | ALA | TYR | LYS | SER | GLU | LEU | GLU | GLU | GLN | ||||
| 10 | LEU | THR | PRO | VAL | ALA | GLU | GLU | THR | ARG | ALA | ||||
| 11 | ARG | LEU | SER | LYS | GLU | LEU | GLN | ALA | ALA | GLN | ||||
| 12 | ALA | ARG | LEU | GLY | ALA | ASP | MET | GLU | ASP | VAL | ||||
| 13 | CYS | GLY | ARG | LEU | VAL | GLN | TYR | ARG | GLY | GLU | ||||
| 14 | VAL | GLN | ALA | MET | LEU | GLY | GLN | SER | THR | GLU | ||||
| 15 | GLU | LEU | ARG | VAL | ARG | LEU | ALA | SER | HIS | LEU | ||||
| 16 | ARG | LYS | LEU | ARG | LYS | ARG | LEU | LEU | ARG | ASP | ||||
| 17 | ALA | ASP | ASP | LEU | GLN | LYS | ARG | LEU | ALA | VAL | ||||
| 18 | TYR | GLN | ALA | GLY | ALA | ARG | GLU | GLY | ALA | GLU | ||||
| 19 | ARG | GLY | LEU | SER | ALA | ILE | ARG | GLU | ARG | LEU | ||||
| 20 | GLY | PRO | LEU | VAL | GLU | GLN | GLY | ARG | VAL | ARG | ||||
| 21 | ALA | ALA | THR | VAL | GLY | SER | LEU | ALA | GLY | GLN | ||||
| 22 | PRO | LEU | GLN | GLU | ARG | ALA | GLN | ALA | TRP | GLY | ||||
| 23 | GLU | ARG | LEU | ARG | ALA | ARG | MET | GLU | GLU | MET | ||||
| 24 | GLY | SER | ARG | THR | ARG | ASP | ARG | LEU | ASP | GLU | ||||
| 25 | VAL | LYS | GLU | GLN | VAL | ALA | GLU | VAL | ARG | ALA | ||||
| 26 | LYS | LEU | GLU | GLU | GLN | ALA | GLN | GLN | ILE | ARG | ||||
| 27 | LEU | GLN | ALA | GLU | ALA | ALA | GLN | ALA | ARG | LEU | ||||
| 28 | LYS | SER | ARG | PHE | GLU | PRO | LEU | ALA | GLU | ASP | ||||
| 29 | MET | GLN | ARG | GLN | TRP | ALA | GLY | GLN | VAL | GLU | ||||
| 30 | LYS | VAL | GLN | ALA | ALA | GLU | GLY | THR | SER | ALA | ||||
| 31 | ALA | PRO | VAL | PRO | SER | ASP | ASN | HIS |
Samples:
sample_1: Apolipoprotein E, [U-100% 13C; U-100% 15N; 80% 2H], 1 mM; phosphate buffer 25 mM; NaCl 25 mM; NaN3 0.05 mM; D2O 5%; DTT-d10 10 mM; EDTA 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG37412 BAG72927 |
| EMBL | CAA25017 |
| GB | AAB59397 AAB59518 AAB59546 AAD02505 AAG27089 |
| PRF | 1506383A |
| REF | NP_000032 NP_001289617 NP_001289618 NP_001289619 NP_001289620 |
| SP | P02649 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts