BMRB Entry 16008
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16008
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Title: Solution structure of the SARS-unique domain-C from the nonstructural protein 3 (nsp3) of the severe acute respiratory syndrome coronavirus PubMed: 19828617
Deposition date: 2008-10-28 Original release date: 2009-01-06
Authors: Johnson, Margaret; Mohanty, Biswaranjan; Pedrini, Bill; Serrano, Pedro; Chatterjee, Amarnath; Herrmann, Torsten; Joseph, Jeremiah; Saikatendu, Kumar; Buchmeier, Michael; Kuhn, Peter; Wuthrich, Kurt
Citation: Serrano, Pedro; Johnson, Margaret; Chatterjee, Amarnath; Neuman, Benjamin; Joseph, Jeremiah; Buchmeier, Michael; Kuhn, Peter; Wuthrich, Kurt. "Nuclear magnetic resonance structure of the nucleic acid-binding domain of severe acute respiratory syndrome coronavirus nonstructural protein 3." J. Virol. 83, 12998-13008 (2009).
Assembly members:
SUD-C, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 227984 Superkingdom: virus Kingdom: not available Genus/species: Coronavirus SARS coronavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SUD-C: GSEEHFVETVSLAGSYRDWS
YSGQRTELGVEFLKRGDKIV
YHTLESPVEFHLDGEVLSLD
KLKSLLS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 466 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SUD-C | 1 |
Entities:
Entity 1, SUD-C 67 residues - Formula weight is not available
| 1 | GLY | SER | GLU | GLU | HIS | PHE | VAL | GLU | THR | VAL | ||||
| 2 | SER | LEU | ALA | GLY | SER | TYR | ARG | ASP | TRP | SER | ||||
| 3 | TYR | SER | GLY | GLN | ARG | THR | GLU | LEU | GLY | VAL | ||||
| 4 | GLU | PHE | LEU | LYS | ARG | GLY | ASP | LYS | ILE | VAL | ||||
| 5 | TYR | HIS | THR | LEU | GLU | SER | PRO | VAL | GLU | PHE | ||||
| 6 | HIS | LEU | ASP | GLY | GLU | VAL | LEU | SER | LEU | ASP | ||||
| 7 | LYS | LEU | LYS | SER | LEU | LEU | SER |
Samples:
sample_1: SUD-C, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 20 mM; sodium phosphate 20 mM; sodium azide 2.8 mM
sample_conditions_1: ionic strength: 0.0628 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 5D APSY-CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 5D APSY-HACACONH | sample_1 | isotropic | sample_conditions_1 |
| 4D APSY-HACANH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.3, Bruker Biospin - collection
MATCH, Volk, Herrmann and Wuthrich - chemical shift assignment
ASCAN, Fiorito, Herrmann, Damberger and Wuthrich - chemical shift assignment
CARA, Keller and Wuthrich - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 16613 |
| PDB | |
| DBJ | BAC81346 BAC81347 BAC81360 BAC81361 BAC81374 |
| GB | AAP13439 AAP13442 AAP13566 AAP13575 AAP30028 |
| REF | NP_828849 NP_828850 NP_828862 |
| SP | P0C6U8 P0C6X7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts