BMRB Entry 16071
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16071
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Title: Mouse prion protein fragment 90-231 at pH 7.0 PubMed: 19546219
Deposition date: 2008-12-19 Original release date: 2009-06-26
Authors: Damberger, Fred; Wuthrich, Kurt; Hornemann, Simone; von Schroetter, Christine
Citation: Hornemann, Simone; von Schroetter, Christine; Damberger, Fred; Wuthrich, Kurt. "Prion protein-detergent micelle interactions studied by NMR in solution." J. Biol. Chem. 284, 22713-22721 (2009).
Assembly members:
mPrP90, polymer, 144 residues, 164114.1 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
mPrP90: GSGQGGGTHNQWNKPSKPKT
NLKHVAGAAAAGAVVGGLGG
YMLGSAMSRPMIHFGNDWED
RYYRENMYRYPNQVYYRPVD
QYSNQNNFVHDCVNITIKQH
TVTTTTKGENFTETDVKMME
RVVEQMCVTQYQKESQAYYD
GRRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mPrP90 | 1 |
Entities:
Entity 1, mPrP90 144 residues - 164114.1 Da.
| 1 | GLY | SER | GLY | GLN | GLY | GLY | GLY | THR | HIS | ASN | ||||
| 2 | GLN | TRP | ASN | LYS | PRO | SER | LYS | PRO | LYS | THR | ||||
| 3 | ASN | LEU | LYS | HIS | VAL | ALA | GLY | ALA | ALA | ALA | ||||
| 4 | ALA | GLY | ALA | VAL | VAL | GLY | GLY | LEU | GLY | GLY | ||||
| 5 | TYR | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | ||||
| 6 | MET | ILE | HIS | PHE | GLY | ASN | ASP | TRP | GLU | ASP | ||||
| 7 | ARG | TYR | TYR | ARG | GLU | ASN | MET | TYR | ARG | TYR | ||||
| 8 | PRO | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | ||||
| 9 | GLN | TYR | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ||||
| 10 | ASP | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | ||||
| 11 | THR | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | ||||
| 12 | PHE | THR | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ||||
| 13 | ARG | VAL | VAL | GLU | GLN | MET | CYS | VAL | THR | GLN | ||||
| 14 | TYR | GLN | LYS | GLU | SER | GLN | ALA | TYR | TYR | ASP | ||||
| 15 | GLY | ARG | ARG | SER |
Samples:
sample_1: mPrP90, [U-99% 13C; U-99% 15N], 1.2 ± 0.05 mM; H2O 90%; D2O 10%; Sodium Phosphate buffer at pH 7.0 5 mM
sample_conditions_1: ionic strength: 0.03 M; pH: 7.0; pressure: 1 atm; temperature: 293.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.8, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts