BMRB Entry 16422
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16422
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Title: Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis PubMed: 17059825
Deposition date: 2009-07-23 Original release date: 2010-03-08
Authors: Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet
Citation: Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet. "Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis" J. Mol. Biol. 1, 109-122 (2007).
Assembly members:
NRho, polymer, 70 residues, 407.568 Da.
C16PN, non-polymer, 407.568 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NRho: MSAVQVLKFPLSVDLAGFVG
LLRRLNVPHRVSEESGQQVL
WVPDERLAEQVRELYRRYPE
GDPQATLEAA
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NRho | 1 |
| 2 | C16PN | 2 |
Entities:
Entity 1, NRho 70 residues - 407.568 Da.
| 1 | MET | SER | ALA | VAL | GLN | VAL | LEU | LYS | PHE | PRO | |
| 2 | LEU | SER | VAL | ASP | LEU | ALA | GLY | PHE | VAL | GLY | |
| 3 | LEU | LEU | ARG | ARG | LEU | ASN | VAL | PRO | HIS | ARG | |
| 4 | VAL | SER | GLU | GLU | SER | GLY | GLN | GLN | VAL | LEU | |
| 5 | TRP | VAL | PRO | ASP | GLU | ARG | LEU | ALA | GLU | GLN | |
| 6 | VAL | ARG | GLU | LEU | TYR | ARG | ARG | TYR | PRO | GLU | |
| 7 | GLY | ASP | PRO | GLN | ALA | THR | LEU | GLU | ALA | ALA |
Entity 2, C16PN - C16PN - 407.568 Da.
Samples:
sample_1: NRho, [U-15N], 1 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_2: NRho, [U-15N], 0.8 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_3: NRho, [U-15N], 0.6 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_4: NRho, [U-15N], 0.5 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_5: NRho, [U-15N], 0.4 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_6: NRho, [U-15N], 0.3 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_7: NRho, [U-15N], 0.2 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_8: NRho, [U-15N], 0.1 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_9: NRho, [U-15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_10: C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%
sample_conditions_1: temperature: 298 K; pH: 5.5; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_5 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_6 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_7 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_8 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_9 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_10 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAK91039 BAP22307 BAP49934 BAQ38821 BAR66878 |
| EMBL | CAW26702 CCQ87103 CDH70226 CDH76316 CDI91489 |
| GB | AAG06474 ABJ12318 ABR81084 AEO74363 AFM64074 |
| REF | NP_251776 WP_003091333 WP_003104987 WP_003110073 WP_003138531 |