BMRB Entry 16487
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16487
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Title: Backbone 1H, 15N and 13C chemical shift assignments for HCV-1b Core+1/S polypeptide. PubMed: 20067524
Deposition date: 2009-09-09 Original release date: 2010-01-28
Authors: Boumlic, Anissa; Nomine, Yves; Kieffer, Bruno; Trave, Gilles
Citation: Boumlic, Anissa; Nomine, Yves; Charbonnier, Sebastian; Dalagiorgou, Georgia; Vassilaki, Niki; Kieffer, Bruno; Trave, Gilles; Mavromara, Penelope; Orfanoudakis, Georges. "Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein." FEBS J. 277, 774-789 (2010).
Assembly members:
HCV-1b_Core+1/S, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HCV-1b_Core+1/S: GAMAMRAWGGQDGSCHPVAP
GLVGAPMTPGVGRVIWVRSS
IPSRAASPTSWGTFRSSAPP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 157 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit 1 | 1 |
Entities:
Entity 1, unit 1 60 residues - Formula weight is not available
Residues -1 and 0 result from the cloning strategy.
| 1 | GLY | ALA | MET | ALA | MET | ARG | ALA | TRP | GLY | GLY | |
| 2 | GLN | ASP | GLY | SER | CYS | HIS | PRO | VAL | ALA | PRO | |
| 3 | GLY | LEU | VAL | GLY | ALA | PRO | MET | THR | PRO | GLY | |
| 4 | VAL | GLY | ARG | VAL | ILE | TRP | VAL | ARG | SER | SER | |
| 5 | ILE | PRO | SER | ARG | ALA | ALA | SER | PRO | THR | SER | |
| 6 | TRP | GLY | THR | PHE | ARG | SER | SER | ALA | PRO | PRO |
Samples:
sample_1: HCV-1b Core+1/S, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 400 mM; DTT 2 mM; Arginine 50 mM; Glutamic acid 50 mM; D2O 7%; H2O 93%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts