BMRB Entry 16597
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16597
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Title: Influenza Virus Fusion Peptide in Membranes without Cholesterol at pH 5.0 PubMed: 19711890
Deposition date: 2009-11-04 Original release date: 2009-11-09
Authors: Weliky, David
Citation: Sun, Yan; Weliky, David. "C13-C13 Correlation Spectroscopy of Membrane-Associated Influenza Virus Fusion Peptide Strongly Supports a Helix-Turn-Helix Motif and Two Turn Conformations" J. Am. Chem. Soc. 131, 13228-13229 (2009).
Assembly members:
Influenza_Fusion_Peptide, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Influenza Virus Taxonomy ID: 11309 Superkingdom: Viruses Kingdom: not available Genus/species: Influenza Virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Influenza_Fusion_Peptide: GLFGAIAGFIENGWEGMIDG
GGKKKKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 63 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Influenza Virus Fusion Peptide, conformer 1 | 1 |
| 2 | Influenza Virus Fusion Peptide, conformer 2 | 1 |
Entities:
Entity 1, Influenza Virus Fusion Peptide, conformer 1 27 residues - Formula weight is not available
| 1 | GLY | LEU | PHE | GLY | ALA | ILE | ALA | GLY | PHE | ILE | ||||
| 2 | GLU | ASN | GLY | TRP | GLU | GLY | MET | ILE | ASP | GLY | ||||
| 3 | GLY | GLY | LYS | LYS | LYS | LYS | GLY |
Samples:
sample_1: Influenza Fusion Peptide, [U-99% 13C; U-99% 15N], 20 mM; DTPC 400 mM; DTPG 100 mM; HEPES 10 mM; MES 5 mM; H2O 27 M
sample_conditions_1: ionic strength: 5 mM; pH: 5.0; pressure: 1 atm; temperature: 233 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C spin diffusion | sample_1 | solid | sample_conditions_1 |
Software:
NMRPipe, Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian Infinity Plus 400 MHz