BMRB Entry 16616
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16616
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Title: assignment for the I214V variant of rabbit prion protein (91-228) PubMed: 20949107
Deposition date: 2009-11-24 Original release date: 2010-10-13
Authors: Li, Jun; Wen, Yi; Lin, Donghai
Citation: Wen, Yi; Li, Jun; Xiong, Minqian; Peng, Yu; Yao, Wenming; Hong, Jing; Lin, Donghai. "Solution Structure and Dynamics of the I214V Mutant of the Rabbit Prion Protein." PLoS ONE 5, e13273-. (2010).
Assembly members:
prion, polymer, 145 residues, Formula weight is not available
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
prion: MQGGTHNQWGKPSKPKTSMK
HVAGAAAAGAVVGGLGGYML
GSAMSRPLIHFGNDYEDRYY
RENMYRYPNQVYYRPVDQYS
NQNSFVHDCVNITVKQHTVT
TTTKGENFTETDIKIMERVV
EQMCVTQYQQESQAAYQRAH
HHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 362 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 874 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | peptide chain | 1 |
Entities:
Entity 1, peptide chain 145 residues - Formula weight is not available
The first residue (M) and the last six residues (His tag) come from the vector of pET30a.
| 1 | MET | GLN | GLY | GLY | THR | HIS | ASN | GLN | TRP | GLY | ||||
| 2 | LYS | PRO | SER | LYS | PRO | LYS | THR | SER | MET | LYS | ||||
| 3 | HIS | VAL | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ALA | ||||
| 4 | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | MET | LEU | ||||
| 5 | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ILE | HIS | ||||
| 6 | PHE | GLY | ASN | ASP | TYR | GLU | ASP | ARG | TYR | TYR | ||||
| 7 | ARG | GLU | ASN | MET | TYR | ARG | TYR | PRO | ASN | GLN | ||||
| 8 | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | TYR | SER | ||||
| 9 | ASN | GLN | ASN | SER | PHE | VAL | HIS | ASP | CYS | VAL | ||||
| 10 | ASN | ILE | THR | VAL | LYS | GLN | HIS | THR | VAL | THR | ||||
| 11 | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | THR | GLU | ||||
| 12 | THR | ASP | ILE | LYS | ILE | MET | GLU | ARG | VAL | VAL | ||||
| 13 | GLU | GLN | MET | CYS | VAL | THR | GLN | TYR | GLN | GLN | ||||
| 14 | GLU | SER | GLN | ALA | ALA | TYR | GLN | ARG | ALA | HIS | ||||
| 15 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: prion, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; sodium acetate 20 mM
sample_conditions_1: ionic strength: 0.04 M; pH: 4.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| BMRB | 15394 15399 16328 |
| PDB | |
| GB | AAC48697 AAD01554 ABL75505 AEM44421 AEM44422 |
| REF | NP_001075490 XP_008254357 XP_008254358 |
| SP | Q95211 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts