BMRB Entry 16757
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16757
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Title: Location and properties of metal-binding sites on the human prion protein PubMed: 11438695
Deposition date: 2010-03-03 Original release date: 2010-03-24
Authors: Jacksn, Graham; Murray, Ian; Hosszu, Laszlo; Gibbs, Nicholas; Waltho, Johnathan; Clarke, Anthony; Collinge, John
Citation: Jacksn, Graham; Murray, Ian; Hosszu, Laszlo; Gibbs, Nicholas; Waltho, Johnathan; Clarke, Anthony; Collinge, John. "Location and properties of metal-binding sites on the human prion protein" Proc. Natl. Acad. Sci. U.S.A. 98, 8531-8535 (2001).
Assembly members:
PrP^(91-231), polymer, 145 residues, Formula weight is not available
CU, non-polymer, 63.546 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
PrP^(91-231): SDPGQGGGTHSQWNKPSKPK
TNMKHMAGAAAAGAVVGGLG
GYVLGSAMSRPIIHFGSDYE
DRYYRENMHRYPNQVYYRPM
DEYSNQNDFVHNCVNITIKQ
HTVTTTTKGENFTETDVKMM
ERVVEQMCITQYERESQAYY
QRGSS
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PrP^(91-231) | 1 |
| 2 | Cu | 2 |
Entities:
Entity 1, PrP^(91-231) 145 residues - Formula weight is not available
91,S
| 1 | SER | ASP | PRO | GLY | GLN | GLY | GLY | GLY | THR | HIS | ||||
| 2 | SER | GLN | TRP | ASN | LYS | PRO | SER | LYS | PRO | LYS | ||||
| 3 | THR | ASN | MET | LYS | HIS | MET | ALA | GLY | ALA | ALA | ||||
| 4 | ALA | ALA | GLY | ALA | VAL | VAL | GLY | GLY | LEU | GLY | ||||
| 5 | GLY | TYR | VAL | LEU | GLY | SER | ALA | MET | SER | ARG | ||||
| 6 | PRO | ILE | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ||||
| 7 | ASP | ARG | TYR | TYR | ARG | GLU | ASN | MET | HIS | ARG | ||||
| 8 | TYR | PRO | ASN | GLN | VAL | TYR | TYR | ARG | PRO | MET | ||||
| 9 | ASP | GLU | TYR | SER | ASN | GLN | ASN | ASP | PHE | VAL | ||||
| 10 | HIS | ASN | CYS | VAL | ASN | ILE | THR | ILE | LYS | GLN | ||||
| 11 | HIS | THR | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ||||
| 12 | ASN | PHE | THR | GLU | THR | ASP | VAL | LYS | MET | MET | ||||
| 13 | GLU | ARG | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | ||||
| 14 | GLN | TYR | GLU | ARG | GLU | SER | GLN | ALA | TYR | TYR | ||||
| 15 | GLN | ARG | GLY | SER | SER |
Entity 2, Cu - Cu - 63.546 Da.
| 1 | CU |
Samples:
sample_1: PrP^(91-231), [U-15N], 500 uM; Mops 5 mM; CuSO4 1000 uM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX v97, Accelrys Software Inc. - data analysis, processing
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 15676 16743 17714 17756 17780 18426 18550 19268 4402 4434 4641 |
| PDB | |
| DBJ | BAA00011 BAG32276 BAG32277 BAG32278 BAG32279 |
| EMBL | CAA58442 CAG46836 CAG46869 |
| GB | AAA19664 AAA60182 AAA68633 AAB59442 AAB59443 |
| REF | NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902 |
| SP | P04156 P40252 |