BMRB Entry 16851
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16851
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Title: The structure of E-protein activation domain 1 bound to the KIX domain of CBP/p300 elucidates leukemia induction by E2A-PBX1 PubMed: 24682819
Deposition date: 2010-04-08 Original release date: 2012-08-02
Authors: Denis, Christopher; Chitayat, Seth; Plevin, Michael; Liu, Shuang; Spencer, Holly; Ikura, Mitsuhiko; LeBrun, David; Smith, Steven
Citation: Denis, Christopher; Langelaan, David; Kirlin, Alyssa; Chitayat, Seth; Munro, Kim; Spencer, Holly; LeBrun, David; Smith, Steven. "Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300." Nucleic Acids Res. ., .-. (2014).
Assembly members:
KIX, polymer, 87 residues, 10353.954 Da.
ETAD1, polymer, 19 residues, 2021.198 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
KIX: GVRKGWHEHVTQDLRSHLVH
KLVQAIFPTPDPAALKDRRM
ENLVAYAKKVEGDMYESANS
RDEYYHLLAEKIYKIQKELE
EKRRSRL
ETAD1: GSGTDKELSDLLDFSAMFS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 438 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 656 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIX | 1 |
| 2 | ETAD1 | 2 |
Entities:
Entity 1, KIX 87 residues - 10353.954 Da.
| 1 | GLY | VAL | ARG | LYS | GLY | TRP | HIS | GLU | HIS | VAL | ||||
| 2 | THR | GLN | ASP | LEU | ARG | SER | HIS | LEU | VAL | HIS | ||||
| 3 | LYS | LEU | VAL | GLN | ALA | ILE | PHE | PRO | THR | PRO | ||||
| 4 | ASP | PRO | ALA | ALA | LEU | LYS | ASP | ARG | ARG | MET | ||||
| 5 | GLU | ASN | LEU | VAL | ALA | TYR | ALA | LYS | LYS | VAL | ||||
| 6 | GLU | GLY | ASP | MET | TYR | GLU | SER | ALA | ASN | SER | ||||
| 7 | ARG | ASP | GLU | TYR | TYR | HIS | LEU | LEU | ALA | GLU | ||||
| 8 | LYS | ILE | TYR | LYS | ILE | GLN | LYS | GLU | LEU | GLU | ||||
| 9 | GLU | LYS | ARG | ARG | SER | ARG | LEU |
Entity 2, ETAD1 19 residues - 2021.198 Da.
| 1 | GLY | SER | GLY | THR | ASP | LYS | GLU | LEU | SER | ASP | ||||
| 2 | LEU | LEU | ASP | PHE | SER | ALA | MET | PHE | SER |
Samples:
sample_KIX_ETAD1_1: KIX 1.2 mM; ETAD1, [U-13C; U-15N], 0.6 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM
sample_KIX_ETAD1_2: KIX, [U-13C; U-15N], 0.6 mM; ETAD1 1.2 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM
condition_KIX_ETAD1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 2D 1H-13C HSQC | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D CBCA(CO)NH | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D C(CO)NH | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D HNCO | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D HNCACB | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D HCCH-TOCSY | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D H(CCO)NH | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D 1H-13C NOESY | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
| 3D 1H-15N NOESY | sample_KIX_ETAD1_1 | isotropic | condition_KIX_ETAD1 |
Software:
NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
TOPSPIN, Bruker Biospin - collection
NMRView v5.2.2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 18314 18315 18694 18695 16467 |
| PDB | |
| DBJ | BAE06125 BAG65526 BAI45616 BAC26095 BAC38116 BAE21912 BAE21989 BAE25324 |
| GB | AAB28651 AAC08447 AAC51331 AAC51770 AAH72594 AAA42115 AAA58632 AAA60311 AAB32662 AAB62389 |
| PRF | 1923401A 2208360B |
| REF | NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371 NP_001071353 NP_001077431 NP_001077436 NP_001187118 NP_001187247 |
| SP | P45481 Q6JHU9 Q92793 P15884 P51514 Q60722 Q61286 Q99081 |
| TPG | DAA15549 DAA01129 DAA25320 |
| EMBL | CAA46052 CAA62868 CAD89914 CAF92364 CAG00972 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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