BMRB Entry 16904

Name: Backbone assignment of human alpha-synuclein in viscous 2 M glucose solution
Published: 2010-09-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16904

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone assignment of human alpha-synuclein in viscous 2 M glucose solution PubMed: 20872101

Deposition date: 2010-04-28 Original release date: 2010-09-28

Authors: Wu, Kuen-Phon; Baum, Jean

Citation: Wu, Kuen-Phon; Baum, Jean. "Backbone assignment and dynamics of human -synuclein in viscous 2M glucose solution." Biomol. NMR Assignments 5, 43-46 (2011).

Assembly members:
alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:

assigned_chemical_shifts
- synuclein_glucose
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	383
15N chemical shifts	135
1H chemical shifts	134
T2 relaxation values	129

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha-synuclein	1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
2	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
3	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
4	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
5	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
6	GLY	VAL	ALA	THR	VAL	ALA	GLU	LYS	THR	LYS
7	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
8	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	SER	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	LEU
11	GLY	LYS	ASN	GLU	GLU	GLY	ALA	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	ASP	ASN	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

Samples:

13C-15N: alpha-synuclein, [U-100% 13C; U-100% 15N], 0.3 – 0.4 mM; H20 80%; D20 20%; glucose 2 M; phosphate 10 mM; NaCl 138 mM; KCl 2 mM

15N: alpha-synuclein, [U-100% 15N], 0.1 – 0.2 mM; H20 80%; D20 20%; glucose 2 M; phosphate 10 mM; NaCl 138 mM; KCl 2 mM

2_M_glucose_in_PBS: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N	isotropic	2_M_glucose_in_PBS
3D CBCA(CO)NH	13C-15N	isotropic	2_M_glucose_in_PBS
3D HNCO	13C-15N	isotropic	2_M_glucose_in_PBS
3D HNCACB	13C-15N	isotropic	2_M_glucose_in_PBS
3D HN(CA)CO	13C-15N	isotropic	2_M_glucose_in_PBS
2D 1H-15N HSQC	15N	isotropic	2_M_glucose_in_PBS
3D HNN	13C-15N	isotropic	2_M_glucose_in_PBS
T2	15N	isotropic	2_M_glucose_in_PBS

Software:

VNMRJ v2.1, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

BMRB	16300 16302 16342 16543 16546 16547 16548 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB	1XQ8 2KKW
DBJ	BAB29375 BAF82858 BAG73790
EMBL	CAG33339 CAG46454
GB	AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF	NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP	P37840 P61139 P61140 P61142 P61143

Biological Magnetic Resonance Data Bank