BMRB Entry 1696
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR1696
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Title: Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Baldisseri, Donna; Pelton, J.; Sparks, Steven; Torchia, Dennis
Citation: Baldisseri, Donna; Pelton, J.; Sparks, Steven; Torchia, Dennis. "Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy" FEBS Lett. 281, 33-38 (1991).
Assembly members:
micrococcal nuclease, polymer, 156 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: not available Host organism: Escherichia coli
Entity Sequences (FASTA):
micrococcal nuclease: MDPTVYSATSTKKLHKEPXT
LXKAIDGXTVKLMYKGXPMT
FRLLLVDTPEXKXPKKGVEK
YGPXASAFTKKMVENAKKIE
VEFNKGQXTDKYGRGLAXIY
ADGKMVNEALVRQGLAKVAY
XYKPNNTHXXHLRKSEAQAK
KEKLNIXSENDADSGQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 165 |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 341 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | micrococcal nuclease | 1 |
Entities:
Entity 1, micrococcal nuclease 156 residues - Formula weight is not available
| 1 | MET | ASP | PRO | THR | VAL | TYR | SER | ALA | THR | SER | ||||
| 2 | THR | LYS | LYS | LEU | HIS | LYS | GLU | PRO | X | THR | ||||
| 3 | LEU | X | LYS | ALA | ILE | ASP | GLY | X | THR | VAL | ||||
| 4 | LYS | LEU | MET | TYR | LYS | GLY | X | PRO | MET | THR | ||||
| 5 | PHE | ARG | LEU | LEU | LEU | VAL | ASP | THR | PRO | GLU | ||||
| 6 | X | LYS | X | PRO | LYS | LYS | GLY | VAL | GLU | LYS | ||||
| 7 | TYR | GLY | PRO | X | ALA | SER | ALA | PHE | THR | LYS | ||||
| 8 | LYS | MET | VAL | GLU | ASN | ALA | LYS | LYS | ILE | GLU | ||||
| 9 | VAL | GLU | PHE | ASN | LYS | GLY | GLN | X | THR | ASP | ||||
| 10 | LYS | TYR | GLY | ARG | GLY | LEU | ALA | X | ILE | TYR | ||||
| 11 | ALA | ASP | GLY | LYS | MET | VAL | ASN | GLU | ALA | LEU | ||||
| 12 | VAL | ARG | GLN | GLY | LEU | ALA | LYS | VAL | ALA | TYR | ||||
| 13 | X | TYR | LYS | PRO | ASN | ASN | THR | HIS | X | X | ||||
| 14 | HIS | LEU | ARG | LYS | SER | GLU | ALA | GLN | ALA | LYS | ||||
| 15 | LYS | GLU | LYS | LEU | ASN | ILE | X | SER | GLU | ASN | ||||
| 16 | ASP | ALA | ASP | SER | GLY | GLN |
Samples:
sample_one:
sample_condition_set_one: pH: 6.8 na; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
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