BMRB Entry 16983
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16983
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Title: The solution structure of the PHD3 finger of MLL PubMed: 20677832
Deposition date: 2010-06-08 Original release date: 2010-08-12
Authors: Park, Sangho; Bushweller, John
Citation: Park, Sangho; Osmers, Ute; Raman, Gayathree; Schwantes, Rebecca; Diaz, Manuel; Bushweller, John. "The PHD3 Domain of MLL Acts as a CYP33-Regulated Switch between MLL-Mediated Activation and Repression ." Biochemistry 49, 6576-6586 (2010).
Assembly members:
PHD3, polymer, 67 residues, 7719.658 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PHD3: GSAKGNFCPLCDKCYDDDDY
ESKMMQCGKCDRWVHSKCEN
LSDEMYEILSNLPESVAYTC
VNCTERH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 262 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 429 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHD3 | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
Entities:
Entity 1, PHD3 67 residues - 7719.658 Da.
| 1 | GLY | SER | ALA | LYS | GLY | ASN | PHE | CYS | PRO | LEU | ||||
| 2 | CYS | ASP | LYS | CYS | TYR | ASP | ASP | ASP | ASP | TYR | ||||
| 3 | GLU | SER | LYS | MET | MET | GLN | CYS | GLY | LYS | CYS | ||||
| 4 | ASP | ARG | TRP | VAL | HIS | SER | LYS | CYS | GLU | ASN | ||||
| 5 | LEU | SER | ASP | GLU | MET | TYR | GLU | ILE | LEU | SER | ||||
| 6 | ASN | LEU | PRO | GLU | SER | VAL | ALA | TYR | THR | CYS | ||||
| 7 | VAL | ASN | CYS | THR | GLU | ARG | HIS |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHD3, [U-98% 13C; U-98% 15N], 1 mM; ZINC ION 100 uM; potassium phosphate 25 mM; DTT 3 mM; sodium chloride 50 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 6.9; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_1 | anisotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Varian Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA03407 BAD92745 |
| EMBL | CAA58584 |
| GB | AAA58669 AAA92511 AAB34770 AAM33377 AAQ63624 |
| REF | NP_001074518 NP_005924 XP_003253537 XP_003910832 XP_004052287 |
| SP | Q03164 |
| TPG | DAA22311 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts