BMRB Entry 17003
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17003
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Title: Vpr(1-20) Vpr(21-40) Vpr(25-40) PubMed: 20920334
Deposition date: 2010-06-16 Original release date: 2010-10-14
Authors: Fossen, Torgils; Solbak, Sara
Citation: Solbak, Sara; Reksten, Tove; Wray, Victor; Bruns, Karsten; Horvli, Ole; Raae, Arnt; Henklein, Petra; Henklein, Peter; Roder, Rene; Mitzner, David; Schubert, Ulrich; Fossen, Torgils. "The intriguing Cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding." BMC Struct. Biol. 10, 31-31 (2010).
Assembly members:
N-terminal_Vpr_peptides, polymer, 40 residues, Formula weight is not available
Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: virus Kingdom: not available Genus/species: Lentivirus HIV
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
N-terminal_Vpr_peptides: MEQAPEDQGPQREPYNEWTL
ELLEELKSEAVRHFPRIWLH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 316 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Vpr(1-40) | 1 |
| 2 | Vpr(25-40) | 1 |
Entities:
Entity 1, Vpr(1-40) 40 residues - Formula weight is not available
| 1 | MET | GLU | GLN | ALA | PRO | GLU | ASP | GLN | GLY | PRO | |
| 2 | GLN | ARG | GLU | PRO | TYR | ASN | GLU | TRP | THR | LEU | |
| 3 | GLU | LEU | LEU | GLU | GLU | LEU | LYS | SER | GLU | ALA | |
| 4 | VAL | ARG | HIS | PHE | PRO | ARG | ILE | TRP | LEU | HIS |
Samples:
sample_1: N-terminal Vpr peptides1 – 2 mM; Aqueous Phosphate Buffer 90%; D2O 10%
sample_conditions_1: ionic strength: 0.050 M; pH: 7; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz