BMRB Entry 17011
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17011
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Title: s34r structure PubMed: 21147916
Deposition date: 2010-06-21 Original release date: 2012-04-10
Authors: Montserret, Roland; Dubuisson, Jean; Penin, Francois
Citation: Albecka, Anna; Montserret, Roland; Krey, Thomas; Tarr, Alexander; Diesis, Eric; Ball, Jonathan; Descamps, Veronique; Duverlie, Gilles; Rey, Felix; Penin, Francois; Dubuisson, Jean. "Identification of new functional regions in hepatitis C virus envelope glycoprotein E2" J. Virol. 85, 1777-1792 (2011).
Assembly members:
s34r, polymer, 36 residues, 4031.715 Da.
Natural source: Common Name: hepatitris C virus Taxonomy ID: 11103 Superkingdom: virus Kingdom: not available Genus/species: Hepacivirus hepatitris C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
s34r: SDLPALSTGLLHLHQNIVDV
QYMYGLSPAITKYVVR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 125 |
| 1H chemical shifts | 263 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | s34r | 1 |
Entities:
Entity 1, s34r 36 residues - 4031.715 Da.
| 1 | SER | ASP | LEU | PRO | ALA | LEU | SER | THR | GLY | LEU | ||||
| 2 | LEU | HIS | LEU | HIS | GLN | ASN | ILE | VAL | ASP | VAL | ||||
| 3 | GLN | TYR | MET | TYR | GLY | LEU | SER | PRO | ALA | ILE | ||||
| 4 | THR | LYS | TYR | VAL | VAL | ARG |
Samples:
sample_1: trifluoroethanol 50%; H2O 50%; DSS 0.01%; s34r 2 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz