BMRB Entry 17055
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17055
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Title: Binding Site Structure of One LRP-RAP Complex Implications for a Common Ligand-Receptor Binding Motif PubMed: 16938309
Deposition date: 2010-07-13 Original release date: 2010-09-08
Authors: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thogersen, Hans; O'Shea, Charlotte; Poulsen, Flemming; Kragelund, Birthe
Citation: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thogersen, Hans; O'Shea, Charlotte; Poulsen, Flemming; Kragelund, Birthe. "Binding Site Structure of One LRP-RAP Complex: Implications for a Common Ligand-Receptor Binding Motif" J. Mol. Biol. 362, 700-716 (2006).
Assembly members:
CR56, polymer, 82 residues, Formula weight is not available
RAPd1, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CR56: SARTCPPNQFSCASGRCIPI
SWTCDLDDDCGDRSDESASC
AYPTCFPLTQFTCNNGRCIN
INWRCDNDNDCGDNSDEAGC
SH
RAPd1: GEEFRMEKLNQLWEKAQRLH
LPPVRLAELHADLKIQERDE
LAWKKLKLDGLDEDGEKEAR
LIRNLNVILAKYGLDGKKDA
R
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CR56 | 1 |
| 2 | RAPd1 | 2 |
Entities:
Entity 1, CR56 82 residues - Formula weight is not available
| 1 | SER | ALA | ARG | THR | CYS | PRO | PRO | ASN | GLN | PHE | ||||
| 2 | SER | CYS | ALA | SER | GLY | ARG | CYS | ILE | PRO | ILE | ||||
| 3 | SER | TRP | THR | CYS | ASP | LEU | ASP | ASP | ASP | CYS | ||||
| 4 | GLY | ASP | ARG | SER | ASP | GLU | SER | ALA | SER | CYS | ||||
| 5 | ALA | TYR | PRO | THR | CYS | PHE | PRO | LEU | THR | GLN | ||||
| 6 | PHE | THR | CYS | ASN | ASN | GLY | ARG | CYS | ILE | ASN | ||||
| 7 | ILE | ASN | TRP | ARG | CYS | ASP | ASN | ASP | ASN | ASP | ||||
| 8 | CYS | GLY | ASP | ASN | SER | ASP | GLU | ALA | GLY | CYS | ||||
| 9 | SER | HIS |
Entity 2, RAPd1 81 residues - Formula weight is not available
| 1 | GLY | GLU | GLU | PHE | ARG | MET | GLU | LYS | LEU | ASN | ||||
| 2 | GLN | LEU | TRP | GLU | LYS | ALA | GLN | ARG | LEU | HIS | ||||
| 3 | LEU | PRO | PRO | VAL | ARG | LEU | ALA | GLU | LEU | HIS | ||||
| 4 | ALA | ASP | LEU | LYS | ILE | GLN | GLU | ARG | ASP | GLU | ||||
| 5 | LEU | ALA | TRP | LYS | LYS | LEU | LYS | LEU | ASP | GLY | ||||
| 6 | LEU | ASP | GLU | ASP | GLY | GLU | LYS | GLU | ALA | ARG | ||||
| 7 | LEU | ILE | ARG | ASN | LEU | ASN | VAL | ILE | LEU | ALA | ||||
| 8 | LYS | TYR | GLY | LEU | ASP | GLY | LYS | LYS | ASP | ALA | ||||
| 9 | ARG |
Samples:
sample_1: CR56, [U-15N], 20 uM; RAPd10 – 40 uM; sodium chloride 100 mM; calcium chloride 10 mM; magnesium chloride 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NACCESS, Hubbard, S. J. and Thornton, J. M. (1993) - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PRONTO3D, Kj r, M., Andersen, K. V. & Poulsen, F. M. (1994). - data analysis
NMR spectrometers:
- Varian Unity 750 MHz
- Varian Unity 800 MHz
Related Database Links:
| PDB | |
| BMRB | 16454 5598 |
| DBJ | BAD96224 BAD96247 BAE01566 BAE87201 BAF85010 |
| EMBL | CAH90295 |
| GB | AAA51553 AAC67373 AAI05075 AAI12068 AAY18903 |
| REF | NP_001125136 NP_001233320 NP_002328 XP_001085674 XP_003279069 |
| SP | P30533 |