BMRB Entry 17170
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17170
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Title: Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis
Deposition date: 2010-09-07 Original release date: 2012-08-01
Authors: Huang, Tai-Huang; Jiang, Ingjye; Tsai, Chen-Kun; Naik, Mandar; Chang, Chi-Fon; Wu, Wen-Jin
Citation: Huang, Tai-huang; Jiang, Ingjye; Tsai, Chen-Kun. "Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis" Not known ., .-..
Assembly members:
tvMyb2_MRE-1, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: Trichomonas vaginalis Taxonomy ID: 5722 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trichomonas vaginalis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
tvMyb2_MRE-1: MVQVNLKAAKKQKFTPEEDE
MLKRAVAQHGSDWKMIAATF
PNRNARQCRDRWKNYLAPSI
SHTPWTAEEDALLVQKIQEY
GRQWAIIAKFFPGRTDIHIK
NRWVTISNKLGIPQTQQMLE
HHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 476 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 723 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tvMyb2 | 1 |
Entities:
Entity 1, tvMyb2 126 residues - Formula weight is not available
| 1 | MET | VAL | GLN | VAL | ASN | LEU | LYS | ALA | ALA | LYS | ||||
| 2 | LYS | GLN | LYS | PHE | THR | PRO | GLU | GLU | ASP | GLU | ||||
| 3 | MET | LEU | LYS | ARG | ALA | VAL | ALA | GLN | HIS | GLY | ||||
| 4 | SER | ASP | TRP | LYS | MET | ILE | ALA | ALA | THR | PHE | ||||
| 5 | PRO | ASN | ARG | ASN | ALA | ARG | GLN | CYS | ARG | ASP | ||||
| 6 | ARG | TRP | LYS | ASN | TYR | LEU | ALA | PRO | SER | ILE | ||||
| 7 | SER | HIS | THR | PRO | TRP | THR | ALA | GLU | GLU | ASP | ||||
| 8 | ALA | LEU | LEU | VAL | GLN | LYS | ILE | GLN | GLU | TYR | ||||
| 9 | GLY | ARG | GLN | TRP | ALA | ILE | ILE | ALA | LYS | PHE | ||||
| 10 | PHE | PRO | GLY | ARG | THR | ASP | ILE | HIS | ILE | LYS | ||||
| 11 | ASN | ARG | TRP | VAL | THR | ILE | SER | ASN | LYS | LEU | ||||
| 12 | GLY | ILE | PRO | GLN | THR | GLN | GLN | MET | LEU | GLU | ||||
| 13 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: EDTA 1 mM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; H2O 93%; D2O 7%; tvMyb2_MRE-1, [U-100% 13C; U-100% 15N], 0.6 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - processing
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts