BMRB Entry 17203
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17203
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Title: NMR structure of Acyl carrier protein from Brucella melitensis. Seattle Structure Genomics Center for Infectious Disease (SSGCID)
Deposition date: 2010-09-23 Original release date: 2010-11-01
Authors: Barnwal, Ravi pratap; Varani, Gabriele
Citation: Barnwal, Ravi Pratap; Varani, Gabriele. "NMR assignment of acyl carrier protein from Brucella melitensis." Not known ., .-..
Assembly members:
entity, polymer, 79 residues, 8395.351 Da.
Natural source: Common Name: Brucella melitensis Taxonomy ID: 29459 Superkingdom: Bacteria Kingdom: not available Genus/species: Brucella melitensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: SMSDTAERVKKIVVEHLGVD
ADKVTEGASFIDDLGADSLD
TVELVMAFEEEFGVEIPDDA
AETILTVGDAVKFIDKASA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 448 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Acyl carrier protein | 1 |
Entities:
Entity 1, Acyl carrier protein 79 residues - 8395.351 Da.
| 1 | SER | MET | SER | ASP | THR | ALA | GLU | ARG | VAL | LYS | ||||
| 2 | LYS | ILE | VAL | VAL | GLU | HIS | LEU | GLY | VAL | ASP | ||||
| 3 | ALA | ASP | LYS | VAL | THR | GLU | GLY | ALA | SER | PHE | ||||
| 4 | ILE | ASP | ASP | LEU | GLY | ALA | ASP | SER | LEU | ASP | ||||
| 5 | THR | VAL | GLU | LEU | VAL | MET | ALA | PHE | GLU | GLU | ||||
| 6 | GLU | PHE | GLY | VAL | GLU | ILE | PRO | ASP | ASP | ALA | ||||
| 7 | ALA | GLU | THR | ILE | LEU | THR | VAL | GLY | ASP | ALA | ||||
| 8 | VAL | LYS | PHE | ILE | ASP | LYS | ALA | SER | ALA |
Samples:
sample_1: acyl carrir protein, [U-98% 15N], 1.2-1.4 mM; H2O 93%; D2O 7%
sample_2: acyl carrir protein, [U-95% 13C; U-95% 15N], 1.2 mM; H2O 93%; D2O 7%
sample_3: acyl carrir protein 1.5 mM; H2O 93%; D2O 7%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAJ10440 CDL75871 |
| GB | AAL52656 AAN29402 AAX73876 ABQ60146 ABS13303 |
| REF | WP_002963616 WP_008039152 WP_018427111 WP_024848545 WP_035033204 |
| SP | A5VP30 A9M8Y2 B0CKE3 B2S9V7 C0RHG7 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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