BMRB Entry 17214
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17214
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: A30P alpha-synuclein fibrils PubMed: 22334684
Deposition date: 2010-09-28 Original release date: 2012-02-20
Authors: Lemkau, Luisel; Comellas, Gemma; Kloepper, Kathryn; Woods, Wendy; George, Julia; Rienstra, Chad
Citation: Lemkau, Luisel; Comellas, Gemma; Kloepper, Kathryn; Woods, Wendy; George, Julia; Rienstra, Chad. "Mutant protein A30P -synuclein adopts wild-type fibril structure, despite slower fibrillation kinetics." J. Biol. Chem. 287, 11526-11532 (2012).
Assembly members:
A30P_alpha-synuclein, polymer, 140 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
A30P_alpha-synuclein: MDVFMKGLSKAKEGVVAAAE
KTKQGVAEAPGKTKEGVLYV
GSKTKEGVVHGVATVAEKTK
EQVTNVGGAVVTGVTAVAQK
TVEGAGSIAAATGFVKKDQL
GKNEEGAPQEGILEDMPVDP
DNEAYEMPSEEGYQDYEPEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 204 |
| 15N chemical shifts | 52 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha-synuclein fibrils | 1 |
Entities:
Entity 1, alpha-synuclein fibrils 140 residues - Formula weight is not available
| 1 | MET | ASP | VAL | PHE | MET | LYS | GLY | LEU | SER | LYS | |
| 2 | ALA | LYS | GLU | GLY | VAL | VAL | ALA | ALA | ALA | GLU | |
| 3 | LYS | THR | LYS | GLN | GLY | VAL | ALA | GLU | ALA | PRO | |
| 4 | GLY | LYS | THR | LYS | GLU | GLY | VAL | LEU | TYR | VAL | |
| 5 | GLY | SER | LYS | THR | LYS | GLU | GLY | VAL | VAL | HIS | |
| 6 | GLY | VAL | ALA | THR | VAL | ALA | GLU | LYS | THR | LYS | |
| 7 | GLU | GLN | VAL | THR | ASN | VAL | GLY | GLY | ALA | VAL | |
| 8 | VAL | THR | GLY | VAL | THR | ALA | VAL | ALA | GLN | LYS | |
| 9 | THR | VAL | GLU | GLY | ALA | GLY | SER | ILE | ALA | ALA | |
| 10 | ALA | THR | GLY | PHE | VAL | LYS | LYS | ASP | GLN | LEU | |
| 11 | GLY | LYS | ASN | GLU | GLU | GLY | ALA | PRO | GLN | GLU | |
| 12 | GLY | ILE | LEU | GLU | ASP | MET | PRO | VAL | ASP | PRO | |
| 13 | ASP | ASN | GLU | ALA | TYR | GLU | MET | PRO | SER | GLU | |
| 14 | GLU | GLY | TYR | GLN | ASP | TYR | GLU | PRO | GLU | ALA |
Samples:
sample_1: A30P alpha-synuclein fibrils, [U-100% 13C; U-100% 15N], 67%; H2O 100%
sample_2: A30P alpha-synuclein fibrils, [U-100% 13C; U-100% 15N], 67%; H2O 100%
sample_conditions_1: pH: 7.5; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NCACX | sample_1 | isotropic | sample_conditions_1 |
| NCOCX | sample_1 | isotropic | sample_conditions_1 |
| CANcoCX | sample_2 | isotropic | sample_conditions_1 |
| CANCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.115, Goddard - chemical shift assignment
NMRPipe v2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian UnityPlus 600 MHz
- Varian UnityPlus 600 MHz