BMRB Entry 17235
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17235
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Title: Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target AnphA.01018.a
Deposition date: 2010-10-02 Original release date: 2010-10-20
Authors: Buchko, Garry
Citation: Buchko, Garry; Hewitt, Stephan; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter. "Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum, the bacterium responsible for human granulocytic ehrlichiosis." Not known ., .-..
Assembly members:
ACP18, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Anaplasma phagocytophilum Taxonomy ID: 948 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anaplasma phagocytophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ACP18: PGSMVSEEIKAQVMESVIGC
LKLNDEQKQILSGTTNLAKD
FNLDSLDFVDLIMSLEERFS
LEISDEDAQKLETVDDICRY
IASKSSDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 370 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 583 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ACP18 | 1 |
Entities:
Entity 1, ACP18 88 residues - Formula weight is not available
| 1 | PRO | GLY | SER | MET | VAL | SER | GLU | GLU | ILE | LYS | ||||
| 2 | ALA | GLN | VAL | MET | GLU | SER | VAL | ILE | GLY | CYS | ||||
| 3 | LEU | LYS | LEU | ASN | ASP | GLU | GLN | LYS | GLN | ILE | ||||
| 4 | LEU | SER | GLY | THR | THR | ASN | LEU | ALA | LYS | ASP | ||||
| 5 | PHE | ASN | LEU | ASP | SER | LEU | ASP | PHE | VAL | ASP | ||||
| 6 | LEU | ILE | MET | SER | LEU | GLU | GLU | ARG | PHE | SER | ||||
| 7 | LEU | GLU | ILE | SER | ASP | GLU | ASP | ALA | GLN | LYS | ||||
| 8 | LEU | GLU | THR | VAL | ASP | ASP | ILE | CYS | ARG | TYR | ||||
| 9 | ILE | ALA | SER | LYS | SER | SER | ASP | ALA |
Samples:
sample_1: ACP18, [U-100% 13C; U-100% 15N], 1 – 2 mM; sodium chloride 100 ± 3 mM; TRIS 20 ± 1 mM; H2O 93%; D2O 7%
sample_2: ACP18, [U-98% 15N], 1 – 2 mM; sodium chloride 100 ± 3 mM; TRIS 20 ± 1 mM; D2O 100%
sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| deuterium exchange | sample_2 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
FELIX v2007, Accelrys Software Inc. - processing
SPARKY v3.115, Goddard - data analysis, peak picking
PSVS v1.1, Bhattacharya and Montelione - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 750 MHz
Related Database Links:
| PDB | |
| GB | ABD44176 AGR78947 AGR80194 AGR81449 EOA61179 |
| REF | WP_044104281 WP_044105205 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts