BMRB Entry 17330
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17330
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Title: NMR solution structure of GIP in micellular media PubMed: 21539943
Deposition date: 2010-12-01 Original release date: 2011-06-01
Authors: Venneti, Kalyana; Alana, Inigo; O'Harte, Finbarr; Malthouse, Paul; Hewage, Chandralal
Citation: Venneti, Kalyana; Malthouse, J. Paul G; Hewage, Finbarr. "Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide." Biochim. Biophys. Acta 1814, 882-888 (2011).
Assembly members:
GIP, polymer, 42 residues, 4990.634 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
GIP: YAEGTFISDYSIAMDKIHQQ
DFVNWLLAQKGKKNDWKHNI
TQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 275 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GIP | 1 |
Entities:
Entity 1, GIP 42 residues - 4990.634 Da.
| 1 | TYR | ALA | GLU | GLY | THR | PHE | ILE | SER | ASP | TYR | ||||
| 2 | SER | ILE | ALA | MET | ASP | LYS | ILE | HIS | GLN | GLN | ||||
| 3 | ASP | PHE | VAL | ASN | TRP | LEU | LEU | ALA | GLN | LYS | ||||
| 4 | GLY | LYS | LYS | ASN | ASP | TRP | LYS | HIS | ASN | ILE | ||||
| 5 | THR | GLN |
Samples:
sample_1: DHPC 150 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.9; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker DRX 800 MHz
- Bruker DRX 900 MHz
Related Database Links:
| BMRB | 17331 |
| PDB | |
| GB | AAA53192 AAA88043 AAH69100 AAH69663 AAH69686 |
| REF | NP_004114 XP_001091383 XP_001172671 XP_002806900 XP_003278797 |
| SP | P09681 |