BMRB Entry 17334
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17334
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Title: EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1 PubMed: 10024441
Deposition date: 2010-12-02 Original release date: 2012-06-06
Authors: Smallridge, Rachel; Whiteman, Pat; Doering, Klaus; Handford, Penny; Downing, A.
Citation: Smallridge, Rachel; Whiteman, Pat; Doering, Klaus; Handford, Penny; Downing, A.. "EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1" J. Mol. Biol. 286, 661-668 (1999).
Assembly members:
EGF, polymer, 86 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EGF: TDIDECRISPDLCGRGQCVN
TPGDFECKCDEGYESGFMMM
KNCMDIDECQRDPLLCRGGV
CHNTEGSYRCECPPGHQLSP
NISACI
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cbEGF12 | 1 |
| 2 | cbEGF13 | 1 |
| 3 | CA | 2 |
Entities:
Entity 1, cbEGF12 86 residues - Formula weight is not available
| 1 | THR | ASP | ILE | ASP | GLU | CYS | ARG | ILE | SER | PRO | ||||
| 2 | ASP | LEU | CYS | GLY | ARG | GLY | GLN | CYS | VAL | ASN | ||||
| 3 | THR | PRO | GLY | ASP | PHE | GLU | CYS | LYS | CYS | ASP | ||||
| 4 | GLU | GLY | TYR | GLU | SER | GLY | PHE | MET | MET | MET | ||||
| 5 | LYS | ASN | CYS | MET | ASP | ILE | ASP | GLU | CYS | GLN | ||||
| 6 | ARG | ASP | PRO | LEU | LEU | CYS | ARG | GLY | GLY | VAL | ||||
| 7 | CYS | HIS | ASN | THR | GLU | GLY | SER | TYR | ARG | CYS | ||||
| 8 | GLU | CYS | PRO | PRO | GLY | HIS | GLN | LEU | SER | PRO | ||||
| 9 | ASN | ILE | SER | ALA | CYS | ILE |
Entity 2, CA - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: EGF 131 uM; sodium chloride 150 mM; Tris 4.55 mM; calcium chloide0 – 277.72 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 306 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
software -
NMR spectrometers:
- GE Omega 600 MHz
Related Database Links:
| PDB |