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BMRB Entry 17383

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17383
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Title: Crimean Congo Hemorrhagic Fever Gn zinc finger   PubMed: 21507948

Deposition date: 2010-12-27 Original release date: 2011-05-03

Authors: Estrada, David; De Guzman, Roberto

Citation: Estrada, D. Fernando; De Guzman, Roberto. "Structural characterization of the crimean-congo hemorrhagic Fever virus gn tail provides insight into virus assembly."  J. Biol. Chem. 286, 21678-21686 (2011).

Assembly members:
CRIMEAN_CONGO_GN_ZINC_FINGER, polymer, 77 residues, 8936.340 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Crimean-Congo hemorrhagic fever virus   Taxonomy ID: 11593   Superkingdom: viruses   Kingdom: not available   Genus/species: nairovirus Crimean-Congo hemorrhagic fever virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CRIMEAN_CONGO_GN_ZINC_FINGER: RELKPQTCTICETTPVNAID AEMHDLNCSYNICPYCASRL TSDGLARHVTQCPKRKEKVE ETELYLNLERIPWVVRK

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts70
1H chemical shifts154
heteronuclear NOE values67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail1
2ZINC ION 12
3ZINC ION 22

Entities:

Entity 1, Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail 77 residues - 8936.340 Da.

1   ARGGLULEULYSPROGLNTHRCYSTHRILE
2   CYSGLUTHRTHRPROVALASNALAILEASP
3   ALAGLUMETHISASPLEUASNCYSSERTYR
4   ASNILECYSPROTYRCYSALASERARGLEU
5   THRSERASPGLYLEUALAARGHISVALTHR
6   GLNCYSPROLYSARGLYSGLULYSVALGLU
7   GLUTHRGLULEUTYRLEUASNLEUGLUARG
8   ILEPROTRPVALVALARGLYS

Entity 2, ZINC ION 1 - Zn - 65.409 Da.

1   ZN

Samples:

cchfv_gn_729-805_n15: CRIMEAN CONGO GN ZINC FINGER, [U-99% 15N], .7 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; H2O 90%; D2O 10%

cchfv_gn_729-805_n15_c13: CRIMEAN CONGO GN ZINC FINGER, [U-99% 13C; U-99% 15N], .5 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; H2O 90%; D2O 10%

cchfv_gn_729-805_n15_c13_D2O: CRIMEAN CONGO GN ZINC FINGER, [U-99% 13C; U-99% 15N], .7 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; D2O 100%

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCcchfv_gn_729-805_n15isotropicsample_conditions_1
3D 1H-15N NOESYcchfv_gn_729-805_n15isotropicsample_conditions_1
3D HNCAcchfv_gn_729-805_n15_c13isotropicsample_conditions_1
3D HNCACBcchfv_gn_729-805_n15_c13isotropicsample_conditions_1
3D HBHA(CO)NHcchfv_gn_729-805_n15_c13isotropicsample_conditions_1
3D CBCA(CO)NHcchfv_gn_729-805_n15_c13isotropicsample_conditions_1
3D 1H-13C NOESYcchfv_gn_729-805_n15_c13_D2Oisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticcchfv_gn_729-805_n15_c13_D2Oisotropicsample_conditions_1
2D 1H-15N HeteroNuclear NOEcchfv_gn_729-805_n15isotropicsample_conditions_1

Software:

CYANA, G ntert P. - refinement

AMBER v7, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB84574 BAB84575 BAB84577 BAB84578
EMBL CAD61343 CAD61345
GB AAA86616 AAK18286 AAM48106 AAM48107 AAP29978
REF NP_950235
SP Q8JSZ3

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