BMRB Entry 17435
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17435
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Title: solution structure of the C-terminal domain of Salmonella H-NS PubMed: 21673140
Deposition date: 2011-01-29 Original release date: 2015-06-23
Authors: Li, Yifei
Citation: Gordon, Blair; Li, Yifei; Cote, Atina; Weirauch, Matthew; Ding, Pengfei; Hughes, Timothy; Navarre, William; Xia, Bin; Liu, Jun. "Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins" Proc. Natl. Acad. Sci. U. S. A. 108, 10690-10695 (2011).
Assembly members:
H-NS, polymer, 49 residues, 5564.294 Da.
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 90371 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
H-NS: AARPAKYSYVDENGETKTWT
GQGRTPAVIKKAMEEQGKQL
EDFLIKELE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 173 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 350 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H-NS | 1 |
Entities:
Entity 1, H-NS 49 residues - 5564.294 Da.
| 1 | ALA | ALA | ARG | PRO | ALA | LYS | TYR | SER | TYR | VAL | ||||
| 2 | ASP | GLU | ASN | GLY | GLU | THR | LYS | THR | TRP | THR | ||||
| 3 | GLY | GLN | GLY | ARG | THR | PRO | ALA | VAL | ILE | LYS | ||||
| 4 | LYS | ALA | MET | GLU | GLU | GLN | GLY | LYS | GLN | LEU | ||||
| 5 | GLU | ASP | PHE | LEU | ILE | LYS | GLU | LEU | GLU |
Samples:
sample_1: C-terminal domain of Salmonella H-NS, [U-13C, >90%; U-15 N, >90%], 0.7 mM; sodium phosphate, [U-100% 13C; U-100% 15N], 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ36716 BAP07615 GAL42187 GAL43811 GAL48349 |
| EMBL | CAA32549 CAD08382 CAR32863 CAR37239 CAR59200 |
| GB | AAB61148 AAL20669 AAO69288 AAV77088 AAX65652 |
| PIR | AC0650 |
| REF | NP_455749 NP_460710 WP_001287382 WP_001287383 WP_001748450 |
| SP | P0A1S2 P0A1S3 Q5PCT5 |
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