BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 17507

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17507

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Title: DJ-1 backbone assignment   PubMed: 18436956

Deposition date: 2011-03-04 Original release date: 2011-03-14

Authors: Eliezer, David; Malgieri, Gaetano

Citation: Malgieri, Gaetano; Eliezer, David. "Structural effects of Parkinson s disease linked DJ-1 mutations"  Protein Sci. 17, 855-868 (2008).

Assembly members:
DJ-1, polymer, 197 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DJ-1: MASKRALVILAKGAEEMETV IPVDVMRRAGIKVTVAGLAG KDPVQCSRDVVICPDASLED AKKEGPYDVVVLPGGNLGAQ NLSESAAVKEILKEQENRKG LIAAICAGPTALLAHEIGFG SKVTTHPLAKDKMMNGGHYT YSENRVEKDGLILTSRGPGT SFEFALAIVEALNGKEVAAQ VKAPLVLKDLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts533
15N chemical shifts171
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DJ-11

Entities:

Entity 1, DJ-1 197 residues - Formula weight is not available

1   METALASERLYSARGALALEUVALILELEU
2   ALALYSGLYALAGLUGLUMETGLUTHRVAL
3   ILEPROVALASPVALMETARGARGALAGLY
4   ILELYSVALTHRVALALAGLYLEUALAGLY
5   LYSASPPROVALGLNCYSSERARGASPVAL
6   VALILECYSPROASPALASERLEUGLUASP
7   ALALYSLYSGLUGLYPROTYRASPVALVAL
8   VALLEUPROGLYGLYASNLEUGLYALAGLN
9   ASNLEUSERGLUSERALAALAVALLYSGLU
10   ILELEULYSGLUGLNGLUASNARGLYSGLY
11   LEUILEALAALAILECYSALAGLYPROTHR
12   ALALEULEUALAHISGLUILEGLYPHEGLY
13   SERLYSVALTHRTHRHISPROLEUALALYS
14   ASPLYSMETMETASNGLYGLYHISTYRTHR
15   TYRSERGLUASNARGVALGLULYSASPGLY
16   LEUILELEUTHRSERARGGLYPROGLYTHR
17   SERPHEGLUPHEALALEUALAILEVALGLU
18   ALALEUASNGLYLYSGLUVALALAALAGLN
19   VALLYSALAPROLEUVALLEULYSASPLEU
20   GLUHISHISHISHISHISHIS

Samples:

sample_1: DJ-1, [U-13C; U-15N; U-2H], 0.3 mM; DTT 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_2: DJ-1, [U-100% 15N], 0.3 mM; DTT 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_3: DJ-1, [U-13C; U-15N], 0.3 mM; DTT 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, processing

VNMR, Varian - collection

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA09603 BAB71781 BAB71782 BAG34938 BAG73513
GB AAC12806 AAH08188 ACJ13695 ADQ32403 EAW71591
REF NP_001116849 NP_009193 XP_001158093 XP_001158489 XP_002750288
SP Q95LI9 Q99497

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts