BMRB Entry 17621
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17621
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Title: Solution structure of the EF-hand domain of Human Polycystin 2
Deposition date: 2011-05-04 Original release date: 2011-12-20
Authors: Allen, Mark; Qamar, S.; Sandford, R.
Citation: Allen, Mark; Qamar, S.; Sandford, R.. "Solution structure of the EF-hand domain of Human Polycystin 2" To Be Published ., .-..
Assembly members:
POLYCYSTIN-2, polymer, 79 residues, 9036.6057 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: unknown
Entity Sequences (FASTA):
POLYCYSTIN-2: GGSLKKNTVDDISESLRQGG
GKLNFDELRQDLKGKGHTDA
EIEAIFTKYDQDGDQELTEH
EHQQMRDDLEKEREDLDLD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 152 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 360 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | POLYCYSTIN-2 | 1 |
| 2 | Calcium Ion | 2 |
Entities:
Entity 1, POLYCYSTIN-2 79 residues - 9036.6057 Da.
| 1 | GLY | GLY | SER | LEU | LYS | LYS | ASN | THR | VAL | ASP | ||||
| 2 | ASP | ILE | SER | GLU | SER | LEU | ARG | GLN | GLY | GLY | ||||
| 3 | GLY | LYS | LEU | ASN | PHE | ASP | GLU | LEU | ARG | GLN | ||||
| 4 | ASP | LEU | LYS | GLY | LYS | GLY | HIS | THR | ASP | ALA | ||||
| 5 | GLU | ILE | GLU | ALA | ILE | PHE | THR | LYS | TYR | ASP | ||||
| 6 | GLN | ASP | GLY | ASP | GLN | GLU | LEU | THR | GLU | HIS | ||||
| 7 | GLU | HIS | GLN | GLN | MET | ARG | ASP | ASP | LEU | GLU | ||||
| 8 | LYS | GLU | ARG | GLU | ASP | LEU | ASP | LEU | ASP |
Entity 2, Calcium Ion - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: POLYCYSTIN-2, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%; Potassium phosphate 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | solution | sample_conditions_1 |
| 2D TOCSY | sample_1 | solution | sample_conditions_1 |
| 2D DQF COSY | sample_1 | solution | sample_conditions_1 |
| 2D HSQC 15N | sample_1 | solution | sample_conditions_1 |
| 2D HSQC 13C | sample_1 | solution | sample_conditions_1 |
| 3D HNCACB | sample_1 | solution | sample_conditions_1 |
| 3D CBCACONH | sample_1 | solution | sample_conditions_1 |
| 3D HNCO | sample_1 | solution | sample_conditions_1 |
| 3D HNCACO | sample_1 | solution | sample_conditions_1 |
| 3D HBHACONH | sample_1 | solution | sample_conditions_1 |
| 3D HNHB | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
CNS v1.2, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| UNP | PKD2_HUMAN |
| BMRB | 16191 16590 18268 |
| PDB | |
| DBJ | BAG56956 BAG57494 |
| EMBL | CAG31243 CAI38797 |
| GB | AAC16004 AAC50520 AAC50933 AAI11455 AAI12262 |
| REF | NP_000288 NP_001026311 NP_001039777 NP_001232908 XP_001099242 |
| SP | Q13563 Q4GZT3 |
| TPG | DAA28806 |
Download simulated HSQC data in one of the following formats:
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