BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 17773

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17773

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Title: Backbone (1)H, (13)C and (15)N NMR chemical shift assignments for the human DGCR8 core   PubMed: 22752847

Deposition date: 2011-07-08 Original release date: 2012-09-20

Authors: Roth, Braden; Hennig, Mirko

Citation: Roth, Braden; Hennig, Mirko. "Backbone HN, 13C, and 15N resonance assignments of the tandem RNA-binding domains of human DGCR8."  Biomol. NMR Assignments 7, 183-186 (2013).

Assembly members:
DGCR8(core), polymer, 214 residues, 24192.8369 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DGCR8(core): SVQDAPTKKEFVINPNGKSE VCILHEYMQRVLKVRPVYNF FECENPSEPFGASVTIDGVT YGSGTASSKKLAKNKAARAT LEILIPDFVKQTSEEKPKDS EELEYFNHISIEDSRVYELT SKAGLLSPYQILHECLKRNH GMGDTSIKFEVVPGKNQKSE YVMACGKHTVRGWCKNKRVG KQLASQKILQLLHPHVKNWG SLLRMYGRESSKMV

Data sets:
Data typeCount
13C chemical shifts590
15N chemical shifts197
1H chemical shifts197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DGCR8(core)1

Entities:

Entity 1, DGCR8(core) 214 residues - 24192.8369 Da.

1   SERVALGLNASPALAPROTHRLYSLYSGLU
2   PHEVALILEASNPROASNGLYLYSSERGLU
3   VALCYSILELEUHISGLUTYRMETGLNARG
4   VALLEULYSVALARGPROVALTYRASNPHE
5   PHEGLUCYSGLUASNPROSERGLUPROPHE
6   GLYALASERVALTHRILEASPGLYVALTHR
7   TYRGLYSERGLYTHRALASERSERLYSLYS
8   LEUALALYSASNLYSALAALAARGALATHR
9   LEUGLUILELEUILEPROASPPHEVALLYS
10   GLNTHRSERGLUGLULYSPROLYSASPSER
11   GLUGLULEUGLUTYRPHEASNHISILESER
12   ILEGLUASPSERARGVALTYRGLULEUTHR
13   SERLYSALAGLYLEULEUSERPROTYRGLN
14   ILELEUHISGLUCYSLEULYSARGASNHIS
15   GLYMETGLYASPTHRSERILELYSPHEGLU
16   VALVALPROGLYLYSASNGLNLYSSERGLU
17   TYRVALMETALACYSGLYLYSHISTHRVAL
18   ARGGLYTRPCYSLYSASNLYSARGVALGLY
19   LYSGLNLEUALASERGLNLYSILELEUGLN
20   LEULEUHISPROHISVALLYSASNTRPGLY
21   SERLEULEUARGMETTYRGLYARGGLUSER
22   SERLYSMETVAL

Samples:

sample_1: DGCR8(core), [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 mM; H2O 90%; D2O 10%

DGCR8_buffer: ionic strength: 0.150 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicDGCR8_buffer
3D HNCACBsample_1isotropicDGCR8_buffer
HNCOCACB (H[N[co[{CA|ca[C]}]]])sample_1isotropicDGCR8_buffer
3D HNCAsample_1isotropicDGCR8_buffer
3D HNCAsample_1isotropicDGCR8_buffer
HSQCNOESYTROSY (h[N]_H[N].NOESY)sample_1isotropicDGCR8_buffer
3D 1H-15N NOESYsample_1isotropicDGCR8_buffer
3D HNCOsample_1isotropicDGCR8_buffer
HNCACO (HNcaCO)sample_1isotropicDGCR8_buffer

Software:

NMRPipe v5.8, Delaglio F, et al - Processing

ANALYSIS v2.1, CCPN - Assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB15165 BAB15238 BAB83032 BAD06468 BAD96516
EMBL CAE46205 CAG30242 CAK54497 CAK54796 CAL37531
GB AAH09323 AAH62919 AAH66118 AAI50110 AAO86726
REF NP_001094674 NP_001099335 NP_001181679 NP_001193848 NP_073557
SP A6QR44 Q8WYQ5 Q9EQM6
TPG DAA20404

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts